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1 March 2014 Phenotypic analysis of an MgtE magnesium transporter mutation in Bacillus subtilis
Sarah Beagle, Corey Suelter, Andrew F. Herbig
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Magnesium (Mg2 ) is the most abundant divalent cation in living cells and plays structural and biochemical roles in many cell processes. Although the importance of Mg2 as a cellular nutrient has been well established, the process by which organisms obtain Mg2 from their environment is still unclear. The Gram-positive bacterium Bacillus subtilis is used as a model system to understand Mg2 uptake and utilization in bacteria. The B. subtilis genome codes for a homolog of the MgtE family of Mg2 transporters. The protein is 34% identical to the structurally well-characterized MgtE homolog in Thermus thermophilus. This study was designed to characterize phenotypes of a B. subtilis mutant deleted for the mgtE gene. Compared to wild type, the ΔmgtE strain exhibits a Mg2 -dependent growth defect, consistent with a role of MgtE in Mg2 transport. Additionally, mutant cells are more resistant to manganese, show a decrease in sporulation efficiency, and are more sensitive to hydrogen peroxide than wild type cells.

Sarah Beagle, Corey Suelter, and Andrew F. Herbig "Phenotypic analysis of an MgtE magnesium transporter mutation in Bacillus subtilis," BIOS 85(1), 8-17, (1 March 2014).
Received: 3 September 2012; Accepted: 1 January 2013; Published: 1 March 2014

metal ion homeostasis
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