Normal embryonic development requires the coordinated expression of many genes. The temporal and spatial expression of these genes is regulated by transcription factors. The LIM homeodomain (LIM-HD) family of transcription factors plays important roles in the formation of the nervous system, reproductive tract, and kidney. lhx1 is a member of the LIM-HD family and is expressed in zebrafish kidney stem cells during development and regeneration. Its interaction with ldb1 has been shown to be important for its activation. However, no other protein is known to interact with lhx1. In this report, we set out to identify new interacting proteins and discovered for the first time that lhx1 can interact with itself to form a dimer. This provides a better understanding of the molecular function of lhx1 and may contribute to designing therapies for diseases caused by lhx1 malfunction.