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1 March 2018 A bioassay for monitoring the effect of diet on lipid accumulation in Drosophila melanogaster larvae
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Abstract
Drosophila is an emerging model organism in which to study the effects of diet on the regulation of metabolism and physiological function. In order to analyze the effects that different diets and food additives have on the accumulation of lipids in Drosophila larvae, we have established a control diet and optimized an established method to monitor the accumulation of lipids in third instar Drosophila larvae known as the floating larvae assay. We report on the simplicity and utility of using the floating larvae assay to access changes in lipid accumulation in flies raised on different diets.
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Samuel L. Goodroad, Alejandra Nunez Torres, Shane McFarlin, Cary Chevalier and Melissa Daggett "A bioassay for monitoring the effect of diet on lipid accumulation in Drosophila melanogaster larvae," BIOS 89(1), (1 March 2018). https://doi.org/10.1893/0005-3155-89.1.9
Received: 26 August 2016; Accepted: 1 July 2017; Published: 1 March 2018
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