The increased prevalence of high-voltage vaping options, where e-liquids are aerosolized at higher battery voltages, has raised concerns regarding the potential health consequences of e-cigarette usage. In this study, rat alveolar type II cells were exposed to vapor generated from unflavored propylene glycol (with and without 24 mg/mL nicotine) and vegetable glycerin e-liquids. Over 30 minutes, L2 cells were exposed to 1800 mL vapor generated at 4.7 V. Cytotoxicity was determined through changes in endocytosis (NRU) and metabolic activity (MTT assay). Level of inflammatory response was determined by prostaglandin E2 (PGE2) secreted into the basolateral cell media post exposure. All values were normalized against control replicates mock-exposed and processed in parallel with experimental replicates. Only cells exposed to propylene glycol without nicotine exhibited significant decreases in cell viability, as measured by NRU.