The objective of the study was to analyse the association of growth hormone (GH1) and C-reactive protein (CRP) loci polymorphisms with reproductive traits in native Pulawska gilts and sows. In the GH1 locus, two mutations were identified: one in the second intron [single-nucleotide polymorphism (SNP) = rs340429823 in.742C>T, MspI], and one in the second exon (SNP = rs340087546 c.566G>A, HaeII). In the CRP locus, two mutations were found in exon 2 (SNP = rs340175625, NM_213844.2: c.1271A>G, BstNI; and SNP = rs80928546, NM_213844.2: c.788C>T, HinfI). Analysis of sexual activity showed that intensity of external estrus signs differed significantly (P ≤ 0.05) and was the most manifested in gilts with the CT (GH1_MspI) genotype during the second estrus. In case of the CRP gene, statistically significant differences (P ≤ 0.05) were found in terms of the duration of farrowing. The longest farrowings were reported for the GG (CRP_HinfI) and the TT (CRP_BstNI) genotypes and the shortest for the AA (CRP_HinfI) and CC (CRP_BstNI). The most numerous first litters were produced by sows with the AA genotype (CRP_HinfI), with significant differences (P ≤ 0.05) between the AA and GG genotypes. In turn, the CC homozygotes (CRP_BstNI) differed significantly (P ≤ 0.05) in terms of the number of piglets born and reared to day 21 in the second litters compared to the other genotype groups.