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1 June 2020 Effects of dietary flaxseed and vitamin E on fermentation, nutrient disappearance, fatty acid biohydrogenation, and microbial protein synthesis using a simulated rumen (Rusitec)
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Abstract

Two simulated rumens (Rusitecs) were used to assess the effects of flaxseed (FS) and (or) vitamin E (VE) on rumen fermentation, fatty acid (FA) biohydrogenation, and microbial protein synthesis. Ground FS replaced 0% or 15% of barley grain, along with VE at 0 or 1000 IU d−1 in a 2 × 2 factorial experiment. Flaxseed lowered neutral detergent fiber (P = 0.001) and acid detergent fiber (P = 0.01) and increased (P = 0.001) nitrogen (N) disappearance. Flaxseed also increased (P = 0.01) total volatile FA and decreased (P = 0.001) acetate production. When both FS and VE were included, the acetate:propionate ratio decreased (P = 0.04). Biohydrogenation of FA was not influenced by VE, but total FA and C18:0 in effluent were increased (P = 0.001) and C16:0 decreased (P = 0.001) by FS. With VE, total microbial N (MN) was increased (P = 0.001). In the concentrate, production of MN in feed-particle-bound bacteria was increased (P = 0.001) by VE. Vitamin E did not alter FA biohydrogenation but did promote MN production. The stable and relatively high pH in the Rusitec may have prevented the typical shift from C18:1 trans-11 to C18:1 trans-10 with concentrate diets. Future studies simulating subclinical acidosis in the Rusitec may illuminate ruminal mode(s) of action of VE on FA biohydrogenation.

Copyright remains with the authors K. Stanford and H. Sultana or their institutions, and © Her Majesty the Queen in right of Canada as represented by the Minister of Agriculture and Agri-Food 2020. Permission for reuse (free in most cases) can be obtained from copyright.com.
K. Stanford, H. Sultana, M.L. He, M. Dugan, and T.A. McAllister "Effects of dietary flaxseed and vitamin E on fermentation, nutrient disappearance, fatty acid biohydrogenation, and microbial protein synthesis using a simulated rumen (Rusitec)," Canadian Journal of Animal Science 100(4), 691-702, (1 June 2020). https://doi.org/10.1139/cjas-2019-0148
Received: 19 August 2019; Accepted: 10 December 2019; Published: 1 June 2020
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