Physiological and behavioral indicators of heat stress in cattle are time- and labor-intensive to measure, and difficult to observe in extensive feedlot and pasture settings. We proposed to record respiration rate and standing behavior using unmanned aerial vehicles. Videos were recorded above steers on feedlot in the morning (0830–1130) and afternoon (1400–1700) over 10 d between 25 July and 10 August and cows on pasture over 9 d between 19 and 29 August In the feedlot, video recordings on 925 individuals (264 black coated, 413 red, and 248 white) were obtained, varying in breed which included Black Angus, Hereford, Charolais, Canadian Speckle Park, and Simmental. On pasture, video recordings on 267 individuals (116 Black Angus and 151 Hereford) were obtained. Observer software was used to analyze videos. Respiration rate in feedlot cattle was the highest in black cattle, followed by red cattle, then white cattle. Coat color did not affect respiration rate in cows on pasture; temperatures on pasture were lower than in feedlots and the effect of coat color may not manifest until a certain heat load threshold. The probability that cattle would be standing increased with heat load index in feedlot and pasture settings.