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24 September 2021 Single nucleotide polymorphism scanning and expression analysis of ACSL1 from different duck breeds
Qianqian Song, Zhixiu Wang, Hongliang Zhang, Xiangxiang Li, Yang Zhang, Qi Xu, Guobin Chang, Hao Zhang, Guohong Chen
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Abstract

Accumulating studies have indicated that the long-chain fatty acyl-CoA1 (ACSL1) gene is related to fat deposition and meat quality in mammals. However, few studies have investigated the relationship between ACSL1 and lipid deposition in ducks. To examine this, we assessed the physicochemical property, homologous alignment, and phylogenetic analyses of the ACSL1 amino acid sequence using bioinformatics tools. The analysis indicated that the ACSL1 amino acid sequence varies in animals, and the duck ACSL1 protein is most closely related to that of chicken. Two single nucleotide polymorphism (SNP) sites were identified at 1749 and 1905 bp of the coding region of ACSL1 by sequencing. Quantitative real-time PCR and western blotting were used to measure mRNA and protein levels in abdominal fat, breast muscle, and liver tissue of Pekin duck (BD) and Cherry Valley duck (CD). mRNA and protein expression were significantly higher in BD than in CD in abdominal fat and liver tissue (P < 0.05). In breast muscle, the mRNA level of ACSL1 was also significantly higher in BD than in CD (P < 0.05), and protein expression in BD tended to be higher than that of CD. These results suggest that ACSL1 may contribute to lipid deposition and meat quality in ducks.

Qianqian Song, Zhixiu Wang, Hongliang Zhang, Xiangxiang Li, Yang Zhang, Qi Xu, Guobin Chang, Hao Zhang, and Guohong Chen "Single nucleotide polymorphism scanning and expression analysis of ACSL1 from different duck breeds," Canadian Journal of Animal Science 102(1), 117-123, (24 September 2021). https://doi.org/10.1139/CJAS-2020-0131
Received: 7 August 2020; Accepted: 2 September 2021; Published: 24 September 2021
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KEYWORDS
ACSL1
ACSL1
Canard
dépôt de lipides
Duck
expression de gènes
gene expression
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