Lu, L., Wang, F., Chen, X., Yuan, H., Tian, Y., Li, J., Shen, J., Tao, Z. and Fu, Y. 2011. cDNA cloning, expression and regulation analysis of goose adipose triglyceride lipase (ATGL) gene. Can. J. Anim. Sci. 91: 363-369. Adipose triglyceride lipase (ATGL) has an important role in adiposome turnover in mammals. In avian species, the ATGL gene has been reported in chicken, duck, quail, turkey and parrot. We describe here the cloning and characterization of the ATGL in goose. Goose ATGL encodes a 482-amino-acid protein, which contains a “GXSXG” motif and 169-amino acid “patatin” domain. The deduced goose ATGL protein shows more than 85% identity to the reported avian species. Quantitative real-time RT-PCR analysis reveals that the goose ATGL mRNA is more highly expressed in subcutaneous fat. We also identify changes of goose ATGL mRNA expression pattern after over-feeding treatment, which may reveal that expression of ATGL in obesity is tissue-specific in goose. Moreover, we conclude that the mRNA level of ATGL can be regulated by oleic acid in goose adipocytes.