Translator Disclaimer
1 December 2011 Short Communication: Rapid separation of cis9, trans11- and trans7,cis9-18:2 (CLA) isomers from ruminant tissue using a 30 m SLB-IL111 ionic column
T. Turner, D. C Rolland, N. Aldai, M. E. R. Dugan
Author Affiliations +
Abstract

Turner, T., Rolland, D. C., Aldai, N. and Dugan, M. E. R. 2011. Short Communication: Rapid separation ofcis9,trans11- andtrans7,cis9-18:2 (CLA) isomers from ruminant tissue using a 30 m SLB-IL111 ionic column. Can. J. Anim. Sci. 91: 711-713. Rumenic acid (cis9,trans11-18:2) is the main natural isomer of conjugated linoleic acid (CLA). Rumenic acid has many purported health benefits, but effects of most other CLA isomers are unknown. Typically trans7,cis9-18:2 is the second most abundant CLA isomer, but it co-elutes with rumenic acid on conventional polar gas chromatography (GC) columns, requiring complimentary analysis with silver-ion high performance liquid chromatography (Ag -HPLC). Herein we report a rapid method for analyzing rumenic acid and trans7,cis9-18:2 using a 30 m ionic-liquid GC column. Optimal resolution of the two CLA isomers was at 145°C and analysis of backfat from barley-fed cattle compared well with GC/Ag -HPLC (y=0.978x - 0.031, r=0.985, P<0.001).

T. Turner, D. C Rolland, N. Aldai, and M. E. R. Dugan "Short Communication: Rapid separation of cis9, trans11- and trans7,cis9-18:2 (CLA) isomers from ruminant tissue using a 30 m SLB-IL111 ionic column," Canadian Journal of Animal Science 91(4), 711-713, (1 December 2011). https://doi.org/10.1139/CJAS2011-071
Received: 23 June 2011; Accepted: 1 August 2011; Published: 1 December 2011
JOURNAL ARTICLE
3 PAGES


Share
SHARE
KEYWORDS
acide linoléique conjugaé
Biohydrogenation
Biohydrogenéation
chromatographie gazeuse
conjugated linoleic acid
gas chromatography
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top