Velleman, S. G., Shin, J., Li, X. and Song, Y. 2012. Review: The skeletal muscle extracellular matrix: Possible roles in the regulation of muscle development and growth. Can. J. Anim. Sci. 92: 1-10. Skeletal muscle fibers are surrounded by an extrinsic extracellular matrix environment. The extracellular matrix is composed of collagens, proteoglycans, glycoproteins, growth factors, and cytokines. How the extracellular matrix influences skeletal muscle development and growth is an area that is not completely understood at this time. Studies on myogenesis have largely been directed toward the cellular components and overlooked that muscle cells secrete a complex extracellular matrix network. The extracellular matrix modulates muscle development by acting as a substrate for muscle cell migration, growth factor regulation, signal transduction of information from the extracellular matrix to the intrinsic cellular environment, and provides a cellular structural architecture framework necessary for tissue function. This paper reviews extracellular matrix regulation of muscle growth with a focus on secreted proteoglycans, cell surface proteoglycans, growth factors and cytokines, and the dynamic nature of the skeletal muscle extracellular matrix, because of its impact on the regulation of muscle cell proliferation and differentiation during myogenesis.

Li, Y. Z., Anderson, J. E. and Johnston, L. J. 2012. Animal-related factors associated with piglet mortality in a bedded, group-farrowing system. Can. J. Anim. Sci. 92: 11-20. To understand pre-weaning mortality of piglets in a bedded, group-farrowing system, associations with sow parity, mortality of piglets during the first 24 h after birth, farrowing behavior, cross-fostering, birth weight and gender of piglets were investigated. Sows (n=169, parity 1 to 10) from eight contemporary groups were evaluated. Litter size at birth, dead piglets from birth to weaning, and individual weight of piglets at birth and weaning (27±2.6 d) were recorded. Behavior of 46 focal sows during farrowing was video-recorded. Piglet mortality within litter increased (P<0.001) with increasing parity. Piglets that died before weaning were lighter (P<0.001) at birth than weaned piglets. Mortality of pigs during the first 24 h postpartum was correlated positively with pre-weaning mortality within litter (R²=0.34; P<0.001). Farrowing behaviors were not associated with piglet mortality, and were not different among sows of different parities, or between sows with and without 24 h piglet mortality. Gender of piglets and cross-fostering did not affect piglet mortality. Results indicate that parity, 24 h mortality, and birth weight of piglets were the major animal-related factors associated with piglet mortality in the loose farrowing system studied.

Schwartzkopf-Genswein, K. S., Shah, M. A., Church, J. S., Haley, D. B., Janzen, K., Truong, G., Atkins, R. P. and Crowe, T. J. 2012. A comparison of commonly used and novel electronic techniques for evaluating cattle temperament. Can. J. Anim. Sci. 92: 21-31. The temperament of steers (n=28) was assessed using five quantitative techniques including: flight time, flight distance, electronic (strain-gauge and accelerometer) tests, and three visual scores (VS) made during entry, restraint and exit from a squeeze chute. The objective of this study was to determine the most important predictive parameters based on those measurements and evaluate the relationship between the techniques. Flight time and distance were correlated with exit VS (r=-0.51, and 0.41, P<0.05; n=56), but were not related to restraint VS. Data from strain-gauge and accelerometer sensors were used to generate parameters such as peak response and area under the curve that were correlated with all three VS. Regression models using VS as the dependent variable and a combination of 2 to 5 parameters from the strain-gauge and accelerometer tests as independent variables predicted temperament with values of 29 to 65 or 41 to 57%, respectively. When all techniques, excluding VS, were used as independent variables, model accuracy increased to 72, 81 and 77% for restraint, exit and the sum of all VS, respectively. These findings suggest the objective measures of temperament assessed in this study could be used to identify highly reactive animals.

Cue, R. I., Pietersma, D., Lefebvre, D., Lacroix, R., Wade, K., Pellerin, D., de Passillé, A-M. and Rushen, J. 2012. Growth modeling of dairy heifers in Québec based on random regression. Can. J. Anim. Sci. 92: 33-47. A total of 144 006 weight (calculated from tape girth measurements) and height data records from Québec dairy heifers were analyzed using random regression to estimate growth curve parameters of Ayrshires, Brown Swiss and Holstein animals to permit prediction of individual heifer growth from 0 to 32 mo. There were, on average, 5.15 records per heifer (minimum 3 records, maximum 25 records). The body weight data were analyzed using linear and quadratic fixed and random regressions, with a power-of-the-mean (POM) function to model the residual variance. The POM was 1.2 for Holstein and Ayrshire and slightly less than 1 for Brown Swiss. Estimated body weight at 24 mo was 507, 564, 624 kg, for Ayrshires, Brown Swiss and Holstein, respectively. The height data were analyzed with a Brody, monomolecular non-linear growth curve model. Mature height was estimated to be 148 cm in both Holstein and Ayrshires, and 150 cm in Brown Swiss. Random regression models were shown to be able to predict individual growth, and can be incorporated in decision-support tools to help producers reducing the average age at first calving.

Wójcik, E. and Smalec, E. 2012. Assessment of chromosome instability in geese (Anser anser). Can. J. Anim. Sci. 92: 49-57. The basic test applied in the research of chromosome instability is the test of sister chromatid exchange (SCE). It makes it possible to identify single-and double-strand DNA damage caused by genotoxic factors and those that disrupt DNA damage repair mechanisms. Fragile sites in chromosomes can be found in all organisms. They are chromosome sites showing susceptibility to breakages and discontinuities in specific conditions of cell culture and also following induction with chemical substances. Chromosome instability of Anser anser geese was assessed in the research, focussing on sister chromatid exchange and the identification of fragile sites. The mean SCE/cell was 4.75±1.00. Most SCEs were identified in the proximal part of the chromosomes. Fragile sites were also identified in the chromosomes during the research. Altogether, 138 breakages were observed in the chromosomes. Apart from identifying chromosome damage, the particular instances of damage were located in the chromosomes.

Pouliot, E., Gariépy, C., Thériault, M., Avezard, C., Fortin, J., Simmons, N. J. and Castonguay, F. W. 2012. Effects of low-voltage electrical stimulation and aging on lamb meat quality. Can. J. Anim. Sci. 92: 59-66. The aim of this study was to evaluate the effect of electrical stimulation (ES) and aging time on meat quality of heavy lamb as produced in Quebec. Seventy-six Suffolk-sired crossbred male lambs were slaughtered at a target weight of 50±2 kg. Half of them were electrically stimulated (ES vs. control) at 5-10 min postmortem (21 V; 0.25 A; 60 s). Postmortem pH decline and temperature were monitored. After carcass cutting, longissimus dorsi sections were assigned to aging periods of 1, 3 or 8 d. Temperature decline was the same for both treatments (P=0.749). However, ES carcasses always had a lower pH value than controls during the first 24 h (P<0.001) while the ultimate pH was equivalent (P=0.803). Tenderness, as assessed by either Warner-Bratzler shear force (39 carcasses) or sensory evaluation (35 carcasses) was enhanced by both ES (P<0.001) and aging (P<0.001). At each aging time, tenderness was greater for ES meat. In addition, only 3 d of aging were necessary for ES meat to achieve the tenderness level attained by the controls after 8 d. Sarcomeres were longer (P<0.001) in ES meat than in controls while myofibrillar fragmentation index was not affected by ES treatment (P=0.743). Electrical stimulation also had small effects on color parameters (a*, b* and L*; P<0.01) and flavor (P=0.04). These results provide the first evidence that tenderness of the meat from heavy lambs produced and processed in Quebec could be enhanced by ES, mostly through cold shortening reduction.

Carcangiu, V., Mura, M. C., Bini, P. P., Vacca, G. M., Daga, C. and Luridiana, S. 2012. Can advance of first lambing induced by melatonin implants influence the next lambing time in Sarda breed sheep? Can. J. Anim. Sci. 92: 67-71. In adult sheep, exogenously administered melatonin from continuous slow-release implants has been shown to advance the onset of the breeding season by mimicking the stimulatory effect of short days. The aim of this study was to verify if treatment with one or two melatonin implants was effective in advancing the first conception in Sarda ewe lambs, and if this advance would also be seen in the second lambing too. In the first year, 600 ewe lambs were randomly assigned to groups M, M M and C, each with 200 animals. On Jun. 30, group M received a single implant while group M M received two implants. Group C was untreated. On Aug. 04, 25 rams were introduced in the groups and removed after 70 d. From these 600 animals the 420 head that lambed prior to Mar. 12 were chosen for the second year. These ewes were subdivided into two groups T1 (ewes who lambed between 2007 Jan. 01 and Feb. 10) and T2 (ewes which lambed between 2007 Feb. 11 and Mar. 12). Both in T1 and T2 the distribution of the animals in M, M M and C group was maintained. In the first year, the treated animals lambed earlier (P<0.05) and showed higher numbers of lambed ewes at Feb. 10 (P<0.01) and at March 12 (P<0.05) compared with untreated control animals. The animals that showed an advance in the period of their first conception, also showed, in the second breeding year, an advance in the lambing time (P<0.001). This study provides evidence that the advance of first conception, obtained with melatonin treatment also influenced the reproductive activity in the following breeding season.

Bolarinwa, O. A., Olukosi, O. A. and Adeola, O. 2012. Metabolizable energy value of porcine meat and bone meal for broiler chickens. Can. J. Anim. Sci. 92: 73-78. Ross 308 broiler chickens at 14 d post-hatch were used in a 7-d experiment to determine the metabolizable energy (ME) value of two meat and bone meal (MBM) samples. Three hundred and thirty-six broiler chickens were grouped by weight into eight blocks of seven cages with six birds per cage. The seven diets consisted of a corn-soybean meal reference diet formulated to meet the NRC (1994) requirement, and six test diets. The reference diet contained four energy sources namely corn, soybean meal, corn starch and soy oil. In the test diets, each of the two MBM samples were added to the reference diet at 30, 60 or 90 g kg^-1 diet to partly replace the energy sources such that the ratio of the energy sources to one another was kept constant in all the diets. The ME and nitrogen-corrected ME (MEn) of the MBM samples were determined by the regression method in which the MBM contribution to ME, and MEn of the diets, in kilocalories, were regressed against the amount of MBM intake in grams. Gross energy of MBM1 and MBM2 were 4247 and 4697 kcal kg^-1 DM, respectively. The crude protein, ash, and crude fat contents for MBM1 or MBM2 were 567 or 594, 264 or 211, and 106 or 116 g kg^-1 DM. Addition of MBM linearly decreased (P<0.05) ileal nitrogen digestibility, total tract utilization of DM, energy and nitrogen as well as ME and MEn of the diets. In conclusion, the respective ME and MEn values were 2512 and 2345 kcal kg^-1 DM for MBM1, and 2711 and 2127 kcal kg^-1 DM for MBM2; thus MBM can be included in the diets of broiler chickens at less than 10% to provide energy.

Hassim, H. A., Lourenço, M., Goh, Y. M., Baars, J. J. P. and Fievez, V. 2012. Rumen degradation of oil palm fronds is improved through pre-digestion with white rot fungi but not through supplementation with yeast or enzymes. Can. J. Anim. Sci. 92: 79-87. Rumen fermentation kinetics of oil palm fronds (OPF) supplemented or not with enzymes (Hemicell^® or Allzyme SSF^®) or yeasts (Levucell^®SC or Yea-Sacc^®) were studied through an in vitro gas production test (96 h) (exp. 1). In exp. 2, enzymes were supplemented to OPF pre-treated during 3 or 9 wk with either one of five white rot fungi strains. Yeasts and enzymes were tested both in active and inactive forms, which revealed the most appropriate set-up to distinguish between the rate of supplements as direct contributors to the fermentation substrate vs. stimulators of the fermentation of the basal substrate. In exp 1, addition of active and inactive Yea-Sacc^® increased the apparently rumen degradable carbohydrates (ARDC) by 11%, whereas enzymes did not affect rumen degradability of non-inoculated OPF. Neither yeast nor enzymes influenced the rate of gas production of non-inoculated OPF, except for active Hemicell^® at the low dose. In exp. 2, inoculation of OPF with Ceriporiopsis subvermispora for 3 wk and Lentinula edodes for 9 wk increased ARDC, but additional enzyme supplementation did not further improve ARDC or the rate of gas production.

Legesse, G., Small, J. A., Scott, S. L., Kebreab, E., Crow, G. H., Block, H. C., Robins, C. D., Khakbazan, M. and McCaughey, W. P. 2012. Bioperformance evaluation of various summer pasture and winter feeding strategies for cow-calf production. Can. J. Anim. Sci. 92: 89-102. Bioperformance of two summer pasture and four winter feeding cow-calf production strategies in the western Canadian Parkland was evaluated. Diet composition and animal data were collected over 5 production years. Each production year began with fixed-time artificial insemination (TAI) of cows and turnout of cow-calf pairs (n=288 yr^-1 including 76 primiparous replacement cows) assigned to either alfalfa-grass (AG, n=9 paddocks) or grass (G, n=9 paddocks) pastures until weaning. Post-weaning, pregnant cows (n=240 yr^-1) were assigned to either extended-grazing (EG, n=120) of dormant regrowth of perennial pastures and swathed annual crops, or one of three diets fed in a drylot (DL): hay (HY, n=40), straw/barley (SB, n=40; 70% oat straw:30% steam-rolled barley grain DM), and silage/straw (SS, n=40; 40% barley silage:60% oat straw DM). Common diets were used for all treatment groups between the weaning and winter feeding period, as well as between the pre-calving and summer grazing period. Cow and calf body weight (BW) gains were higher (P<0.05) for AG than G pasture until the third production year and the advantage diminished as the carrying capacity declined. The latter may be attributed to a lack of spring/summer moisture. Further, G pastures required more nitrogen fertilizer to achieve the same level of bioperformance as that of AG pastures in years 4 and 5. Cows in the EG treatment maintained BW better than those in the DL treatment (especially those cows receiving the SS diet) except in year 5 (P<0.05) in which drought resulted in lower body weights for cows in the EG treatment. On all treatments, cows maintained BCS that supported reproductive function; however, fertility to TAI was lowest (P<0.05) in years 4 and 5. Cows in the DL group had a 1.8 times greater risk of being culled before turnout and as a result lower (P<0.05) rates of calf survival to weaning. In conclusion, AG pastures and EG are important alternatives to further develop for cow-calf production in western Canada.