He, M. L., Gibb, D., McKinnon, J. J. and McAllister, T. A. 2013. Effect of high dietary levels of canola meal on growth performance, carcass quality and meat fatty acid profiles of feedlot cattle. Can. J. Anim. Sci. 93: 269-280. This study investigated the effect of substituting canola meal (CM) for barley grain on growth performance, carcass quality and meat fatty acid (FA) profiles of feedlot cattle. Cross bred calves (n=140; 285±27 kg) were individually fed diets comprised of a barley grain based concentrate (including 5% supplement) and barley silage at ratios of 45:55 and 92:8 (DM basis) during growing and finishing periods, respectively. Pressed CM from Brassica napus, containing 11.4% residual oil and solvent-extracted CM derived from B. napus or Brassica juncea canola seed were compared. Canola meal was substituted for 0 (control), 15, or 30% barley grain (DM basis) in both growing and finishing diets. Regardless of diet, cattle did not differ (P>0.05) in average daily gain in either the growing or finishing period. For the overall feeding period, inclusion of 30% CM increased (P<0.01) DMI of cattle compared with 15% CM groups, but reduced (P<0.05) gain: feed (G:F) as compared with control and 15% B. juncea and 15% pressed CM. Gain: feed of cattle fed CM was also reduced (P<0.05) during the finishing period as compared with the control diet with this reduction being more notable at the 30% level. Carcass quality and incidence of liver abscesses were not affected (P>0.05) by inclusion of CM. Inclusion of 30% pressed CM resulted in higher (P<0.05)�ME of total polyunsaturated fatty acid, n-3, alpha-linolenic acid and conjugated linoleic acid (CLA), and a decrease (P<0.05) in n-6/n-3 ratio in the pars costalis diaphragmatis muscle as compared with the control diet. In conclusion, inclusion of CM did not alter the growth performance or G:F of beef cattle during the growing period, but did lower G:F during the finishing period. The inclusion of 15 or 30% solvent-extracted CM did not alter carcass quality, whereas 30% pressed CM increased the levels of desirable fatty acids (i.e., n-3 and CLA) in beef.