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24 August 2015 Spontaneous sister chromatid exchange in mitotic chromosomes of the chinchilla (Chinchilla lanigera)
Marta Kuchta-Gladysz, Ewa Wójcik, Olga Szeleszczuk, Piotr Niedbala, Kaja Tyblewska
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Abstract

Kuchta-Gladysz, M., Wójcik, E., Szeleszczuk, O., Niedbala, P. and Tyblewska, K. 2015. Spontaneous sister chromatid exchange in mitotic chromosomes of the chinchilla (Chinchilla lanigera). Can. J. Anim. Sci. 95: 543-550. The sister chromatid exchange (SCE) test is a cytogenetic tool with applications as a short-term screen. It is used to assess the influence of physical and chemical factors with potential mutagenic and genotoxic properties on the animal organism. The test results make it possible to eliminate mutagens, as well as helping to predict possible genetic consequences in animal cells and assess animal resistance. The mitotic chromosomes were obtained from an in vitro culture of peripheral blood lymphocytes with added bromodeoxyuridine (BrdU), at five different concentrations: 0.25, 0.5, 1.0, 2.5, and 5.0 µg mL-1. The chromosomes were stained according to the FPG method. Our analyses revealed the spontaneous SCE level in the chinchilla at the concentration of 0.5 µg mL-1. Higher concentrations of this substance have a genotoxic effect and cause damage to the DNA structure of the chromosomes by inducing additional SCEs in the chromosomes of this species. The mean SCE/cell incidence in the chinchilla population was 4.34±1.28. We investigated the effects of age on the incidence of SCE and found it significantly affected this phenomenon in both sexes.

Marta Kuchta-Gladysz, Ewa Wójcik, Olga Szeleszczuk, Piotr Niedbala, and Kaja Tyblewska "Spontaneous sister chromatid exchange in mitotic chromosomes of the chinchilla (Chinchilla lanigera)," Canadian Journal of Animal Science 95(4), 543-550, (24 August 2015). https://doi.org/10.1139/cjas-2015-073
Received: 15 April 2015; Accepted: 1 August 2015; Published: 24 August 2015
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KEYWORDS
chinchilla
chinchilla
chromosome instability
chromosome mitotique
échange de chromatides soeurs
instabilité des chromosomes
mitotic chromosome
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