Age-dependent stability of nucleoli and global DNA methylation level in spermatocytes of the domestic horse (Equus caballus)

Katarzyna Andraszek; Magdalena Gryzińska; Agata Danielewicz; Justyna Batkowska; Elżbieta Smalec

doi:10.1139/cjas-2015-0076

Translator Disclaimer

1 April 2016 Age-dependent stability of nucleoli and global DNA methylation level in spermatocytes of the domestic horse (Equus caballus)

Katarzyna Andraszek, Magdalena Gryzińska, Agata Danielewicz, Justyna Batkowska, Elżbieta Smalec

Author Affiliations +

Canadian J. of Animal Science, 96(2):215-220 (2016). https://doi.org/10.1139/cjas-2015-0076

Abstract

The aim of the study was to determine the number and shape of nucleoli during meiosis in cells of the domestic horse. In addition, the level of global DNA methylation was determined using a quantitative technique for measuring the relative level of DNA methylation, modelled on an immunoenzymatic assay. The research was carried out on stallions belonging to two age groups (2 and 7 yr). In the cells of the 2-yr-old animals, the nucleoli were mainly of a regular shape and no fragmented nucleoli were observed. The cells of the 7-yr-old horses had a small percentage of regularly shaped nucleoli, and nucleoli with a fragmented structure were present. The study provides a basis for further research on epigenetic mechanisms in horses.

Copyright remains with the author(s) or their institution(s). Permission for reuse (free in most cases) can be obtained from RightsLink.

Citation Download Citation

Katarzyna Andraszek, Magdalena Gryzińska, Agata Danielewicz, Justyna Batkowska, and Elżbieta Smalec "Age-dependent stability of nucleoli and global DNA methylation level in spermatocytes of the domestic horse (Equus caballus)," Canadian Journal of Animal Science 96(2), 215-220, (1 April 2016). https://doi.org/10.1139/cjas-2015-0076

Received: 16 April 2015; Accepted: 1 February 2016; Published: 1 April 2016

ACCESS THE FULL ARTICLE

PERSONAL SIGN IN
Full access may be available with your subscription

Password Forgot your password?

Show

Remember Email on this computer

Remember Password

No BioOne Complete account? Create an account
or Access Institutional Sign In via Shibboleth or OpenAthens

PURCHASE THIS CONTENT

PURCHASE SINGLE ARTICLE

Price: $35.00 ADD TO CART

Includes PDF & HTML, when available

RIGHTS & PERMISSIONS

Show All Keywords

KEYWORDS/PHRASES

PUBLICATION TITLE:

PUBLICATION YEARS