Myofiber characteristics, R values, and eating quality were studied in Longissimus thoracis (LT), Psoas major (PM), and Semitendinosus (ST) muscles that had been excised from 10 Chinese Simmental bulls. The diameters of type I, IIA, and IIB fibers in PM are the smallest, and the percentage of type I, IIA, and IIB fibers is highest in PM, LT, and ST, respectively. R₂₄₈ and R₂₅₀ values are higher in PM than in LT and ST. R₂₄₈ and R₂₅₈ are negatively correlated to the proportion of type IIA fibers and positively correlated to the area percentage of type I fibers. PM has a lower Warner–Bratzler shear force (WBSF) and hardness values compared with LT and ST. Semitendinosus has a higher L* value than PM. WBSF, hardness, and h values are positively correlated with the diameter of all three fiber types. Warner–Bratzler shear force has a negative relationship with the percentage of type I fibers, while WBSF and hardness are positively correlated with the area percentage of type IIB fibers. The results demonstrate that the muscle fiber type influences postmortem metabolic rate and meat quality of Chinese Simmental cattle. An increase in the percentage of type I fibers and a decrease in the percentage of type IIB fibers correspond to an improvement in meat quality.