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4 August 2016 Apical Na -dependent neutral amino acid exchanger ASCT2/ATB0 and mTOR-signaling components are expressed along the entire jejunal crypt–villus axis in young pigs fed a liquid milk replacer
Chengbo Yang, Xiaojian Yang, Ming Z. Fan
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Abstract

The Na -dependent neutral amino acid (AA) exchanger ASCT2 (ATB0) plays an important role in the apical exchange of large neutral AAs such as glutamine (Gln) and leucine (Leu) and is essential to maintaining gut mucosal growth. The objectives of this study were to investigate expression of the ASCT2/ATB0 (SLC1A5) gene and the key mechanistic (mammalian) target of rapamycin (mTOR) signaling components in epithelia along the jejunal crypt–villus axis in young pigs fed a liquid milk replacer. Reverse transcription polymerase chain reaction (RT-PCR) analyses showed that the ASCT2/ATB0 (SLC1A5) gene mRNA abundance was higher (P < 0.05) in the crypt than in the upper villus and the middle villus cells. There were no differences (P > 0.05) in the ASCT2/ATB0 protein abundances in the cell homogenate, the intracellular fraction, and the apical membrane among the upper villus, middle villus, and crypt cells. Abundance of phosphorylated mTOR (p-mTOR) protein was higher (P < 0.05) in the middle villus than in the upper villus cells. Phosphorylated p70 S6 kinase protein (p-S6K1) abundance was higher (P < 0.05) in the crypt than in the upper villus cells. Thus, the ASCT2/ATB0 (SLC1A5) gene mRNA and protein ATB0 and the key mTOR-signaling components, p-mTOR and p-S6K1, were expressed in the epithelia along the entire jejunal crypt–villus axis in the young pigs fed a liquid milk replacer.

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Chengbo Yang, Xiaojian Yang, and Ming Z. Fan "Apical Na -dependent neutral amino acid exchanger ASCT2/ATB0 and mTOR-signaling components are expressed along the entire jejunal crypt–villus axis in young pigs fed a liquid milk replacer," Canadian Journal of Animal Science 97(1), 19-29, (4 August 2016). https://doi.org/10.1139/cjas-2016-0021
Received: 2 February 2016; Accepted: 1 July 2016; Published: 4 August 2016
JOURNAL ARTICLE
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KEYWORDS
axe crypte–villosités de l’intestin grêle
cible mécanique et de mammifère de la signalisation rapamycine
échangeur 2 d’acides aminées neutres du système ASC dépendant du sodium
jeunes porcs
mechanistic (mammalian) target of rapamycin signaling
small intestinal crypt–villus axis
system ASC sodium-dependent neutral AA exchanger 2
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