Translator Disclaimer
3 August 2017 Effects of alanyl-glutamine supplementation on the small intestinal mucosa barrier in weaned piglets challenged with lipopolysaccharide
Author Affiliations +
Abstract

The objectives of this study were to investigate the effects of alanyl-glutamine (Ala-Gln) dipeptide supplementation on the intestinal mucosa barrier in weaned piglets challenged by lipopolysaccharide (LPS). A total of 96 piglets were arranged in a 2 × 2 factorial design and randomly divided into four groups. The main effects were LPS challenge (0 or 100 μg kg-1 body weight) and diets (0.62% Ala or 0.5% Ala-Gln). The results showed that LPS challenge significantly increased the crypts depth and decreased the villi height of small intestine in the piglets (P < 0.05), whereas, dietary Ala-Gln supplementation increased the villi height of small intestine (P < 0.05). The LPS challenge decreased the mRNA expressions of epidermal growth factor receptor (EGFR) and insulin-like growth factor 1 receptor (IGF-1R) in the jejunal mucosa (P < 0.05), and Ala-Gln supplementation promoted the mRNA expressions of EGFR and IGF-1R. In addition, Ala-Gln supplementation also increased the mRNA and protein expressions of Claudin-1 and zonula occludens protein-1 (P < 0.05), decreased the number of goblet cells in the jejunal and ileal epithelium (P < 0.05) and the mRNA expressions of interleukin-1β and interleukin-8 in the jejunal mucosa (P < 0.05). There was no significant interaction between Ala-Gln supplementation and LPS challenge in most parameters except for the concentrations of the jejunal mucosa immunoglobulins (P < 0.05). The results indicated that Ala-Gln supplementation was beneficial to strengthen the intestinal mucosal barrier in piglets and could partially attenuate the adverse effects caused by LPS challenge.

Copyright remains with the author(s) or their institution(s). Permission for reuse (free in most cases) can be obtained from RightsLink.
S. Xing, B.L. Zhang, M. Lin, J.L. Li, L. Zhang, F. Gao, and G.H. Zhou "Effects of alanyl-glutamine supplementation on the small intestinal mucosa barrier in weaned piglets challenged with lipopolysaccharide," Canadian Journal of Animal Science 98(1), 144-155, (3 August 2017). https://doi.org/10.1139/cjas-2016-0039
Received: 4 March 2016; Accepted: 1 July 2017; Published: 3 August 2017
JOURNAL ARTICLE
12 PAGES


SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top