Fusarium head blight (FHB) is a devastating wheat disease with a significant economic impact. Fhb5 is an important quantitative trait loci (QTL) conferring type I resistance to FHB. In this study, we accessed the usability of a Kompetitive allele-specific polymerase chain reaction (KASP) marker for the QTL on Fhb-5AS. The KASP clustering results were compared with the linked simple sequence repeat marker, wmc705 for Qfhb-5AS. Our results indicate that the single-nucleotide polymorphic locus wsnp_Ra_c24707_34262900 (IWA7777) provides reliable information for Qfhb-5AS-based resistance and would be amenable to masker-assisted selection, introgression of the resistance loci, and pyramiding of FHB resistance in wheat cultivars.
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