BioOne.org will be down briefly for maintenance on 17 December 2024 between 18:00-22:00 Pacific Time US. We apologize for any inconvenience.
Open Access
How to translate text using browser tools
13 September 2021 Phytochrome contributes to blue-light-mediated stem elongation and flower initiation in mature Arabidopsis thaliana plants
Yun Kong, Youbin Zheng
Author Affiliations +
Abstract

To examine whether phytochromes contribute to blue-light-mediated stem elongation, plant phenotypic responses were investigated in wild type Arabidopsis thaliana (Col-0), and its quintuple phytochrome (phyA phyB phyC phyD phyE) mutant plants under the following light treatments: (1) R, a pure red light from 660-nm LED; (2) B, a pure blue light from 455-nm LED; (3) BR, a impure blue light from LED combination of 94% B and 6% R; and (4) BRF, another impure blue light from LED combination of BR and 6 μmol·m−2·s−1 of FR (735 nm). A photosynthetic photon flux density of ≈100 μmol·m−2·s−1 was provided for all the light treatments. The calculated phytochrome photoequilibrium was 0.89, 0.50, 0.69, and 0.60 for R, B, BR, and BRF, respectively, indicating a higher phytochrome activity under R and BR than B and BRF. After 18 days of light treatment, B or BRF increased main stem length in wild-type plants compared with R, but BR had an inhibition effect similar to R. Also, B and BRF relative to R or BR induced earlier flowering and reduced leaf size in wild type plants, showing typical shade-avoidance responses. In phytochrome-deficient mutant plants, the above shade-avoidance responses were inhibited under B or BRF. However, hypocotyl length, a growth trait characterizing the de-etiolation stage, was reduced under B, BR and BRF vs. R regardless of phytochrome absence. These findings suggest that for mature Arabidopsis plants, phytochrome plays a role in blue-light-mediated stem elongation and the associated shade-avoidance responses.

Introduction

Previous studies using broad-band light sources indicated that blue light (BL), compared with red light (RL), inhibited shoot/leaf elongation (Cosgrove 1981; Appelgren 1991; Wheeler et al. 1991; Hoenecke et al. 1992; Brown et al. 1995; Kong et al. 2012). However, in the past decades, studies using light-emitting diode (LED) lighting have reported that stem/leaf elongation was promoted by pure BL, compared with RL, in a wide range of species (Heo et al. 2002; Hirai et al. 2006; Mizuno et al. 2011; Hata et al. 2013; Kim et al. 2014; Schwend et al. 2015; Fukuda et al. 2016; Hernandez and Kubota 2016), despite some exceptions (Chen et al. 2014; Izzo et al. 2020; Vitale et al. 2020). Unlike LED, possibly, the aforementioned non-LED light sources may have provided impure monochromatic light (Bergstrand et al. 2014). For example, the BL from monochromatic fluorescent lamp was reported to contain a low level of other wavelengths, and have a high red/far-red ratio (i.e., 1.87) which may activate phytochromes (Appelgren 1991).

The promotion effects of pure BL on stem elongation have been also found in our recent studies on ornamental plants and microgreens under LED lighting, and these phenomena have been concluded as related to lower phytochrome activity (Kong et al. 2018, 2019a, 2019b, 2020). In these LED studies, pure BL (B) promoted stem elongation compared with RL (R). However, when a small portion (6% or 10%) of R was added to B, the impure BL (BR) reversed the B promotion effect on elongation, and had similar or greater inhibition effect relative to R. After further adding a low level of far-red light (FR) to BR (R/FR ≈ 1), the resulting impure BL (BRF) recovered the promotion effect similar to B, compared with R. The R/FR reversibility is the classic signature of phytochrome action. Also, as an indicator of phytochrome activity, the phytochrome photostationary state (PPS) value was lower for B (0.5) and BRF (0.6) than R (0.9) and BR (0.7). When the PPS value decreases to 0.6, most plant species show an inactive phytochrome response (Stutte 2009). Since B reduces PPS below 0.6, possibly the B-promoted elongation is related to low phytochrome activity, and under certain conditions B might need to co-act with R to inhibit elongation growth by increasing phytochrome activity. However, the speculation about the involvement of phytochrome in BL action was only based on reversal response to R/FR and calculated PPS values. It needs further confirmation from direct evidence such as a comparison of phenotypic responses to the above light treatments between wild Arabidopsis thaliana and its phytochrome mutant plants.

For wild type Arabidopsis, co-action with RL was found to increase BL’s inhibition effect on hypocotyl elongation of de-etiolated seedlings in a previous study (Ahmad and Cashmore 1997), where the inhibitory effect was enhanced by 10 min RL pulses following 10 min BL pulses, but partially reversed by a subsequent 10 min FR pulses. It was concluded that active phytochrome is required for full expression of cryptochrome activity, which mediates BL’s inhibition effect on hypocotyl elongation (Ahmad and Cashmore 1997). However, differing from our recent study on bedding plants, in the study on Arabidopsis by Ahmad and Cashmore (1997), BL alone inhibited hypocotyl elongation relative to RL, and co-action with RL only strengthened the BL’s inhibition effect. The different result about the BL response may be due to different lighting source (non-LED vs. LED), different plant species (Arabidopsis vs. bedding plants), and different growth stage (de-etiolation stage vs. vegetative stage). In this case, for mature plants of wild Arabidopsis under LED lighting treatments similar to our previous studies, whether B and BRF, relative to R and BR, can promote plant elongation similarly to bedding plants needs further study. Also, phytochrome was only shown to be involved in BL’s inhibition effect on plant elongation in the study by Ahmad and Cashmore (1997). However, it is unknown whether phytochrome also contributes to stem elongation promoted by B or BRF from LED lighting in our previous studies.

In contrast to the above opinion that active phytochrome is required for BL-mediated inhibition effect, some earlier studies on phytochrome-deficient Arabidopsis mutants (phyA and phyB) showed little impairment in BL-dependent inhibition of hypocotyl elongation (Koornneef et al. 1980; Young et al. 1992). However, it has been shown that considerable residual phytochrome responses are retained in all the above phytochrome-deficient mutants (Chory et al. 1989; Reed et al. 1994; Ahmad and Cashmore 1997). The phytochrome family in Arabidopsis has five members: phyA, phyB, phyC, phyD, and phyE, and they have partially overlapping functions (Strasser et al. 2010). Although phyA and phyB are the most important two phytochrome family members, the other three members, phyC, phyD, and phyE, can co-action with phyA or phyB to regulate plant growth and development (Legris et al. 2019). For example, phyA, phyB, phyC, phyD, and phyE can regulate seedling de-etiolation; phyA, phyB, and phyE can suppress stem elongation; and phyB, phyD, and phyE can suppress shade avoidance (Franklin and Quail 2010). In this case, the possibility that other phytochrome family members (e.g., phyC, phyD and (or) phyE) may also contribute to BL-mediated inhibition of hypocotyl elongation cannot be ruled out (Strasser et al. 2010). A recent study on the quintuple phytochrome mutant (phyA phyB phyC phyD phyE) indicated that BL alone inhibited hypocotyl elongation of de-etiolated Arabidopsis seedlings, suggesting that cryptochrome can operate in the absence of phytochrome (Strasser et al. 2010). However, in the above studies, the investigation of elongation growth was focused only on hypocotyl length of de-etiolated seedlings and was performed under non-LED lighting which might provide impure BL in many cases. Therefore, the stem elongation response of mature Arabidopsis plants needs to be further tested in quintuple phytochrome mutant under BL from LED lighting.

Our recent studies on bedding plants and microgreens indicate that the plant elongation promoted by B or BRF is a shade-avoidance response (Kong et al. 2018; Kong et al. 2019b). Besides increased stem elongation, B or BRF, relative to R or BR, caused earlier flowering, smaller cotyledon, longer petiole, and lighter leaf greenness, which varied sensitivity among different species. Possibly, under the same BL treatments with low PPS (i.e., B or BRF) as our recent study, there is a similar shade-avoidance response in the wild-type Arabidopsis plants. Since the shade-avoidance response was mediated by BL associated with low phytochrome activity (i.e., B or BRF), it is possible that the quintuple phytochrome mutant may differ from wild type in the response to these BL treatments.

Based on the above information, when the light treatments (R, B, BR, and BRF) similar to our recent study on bedding plants were used for wild-type Arabidopsis and quintuple phytochrome mutant, three hypotheses were proposed as follows: (1) wild-type plants show an elongation response pattern similar to bedding plants; (2) quintuple phytochrome mutants differ from wild type in their elongation responses to light treatments; (3) B or BRF, compared with R or BR, can induce some shade-avoidance responses in the wild-type, but quintuple phytochrome mutant can change this response. The objective of this study was to explore the involved mechanism for BL action by testing the above hypotheses.

Materials and Methods

Plant materials and maintenance

The experiment was performed at the University of Guelph, Guelph, ON, Canada. Two genotypes of Arabidopsis, wild type (Col-0) and quintuple phytochrome (phyA phyB phyC phyD phyE) mutant (Strasser et al. 2010), were used for this experiment. Taking into account the low seed germination capacity of this phytochrome-deficient mutant, before seeding, seeds were suspended in GA4+7 (Duchefa Biochemie, Haarlem, the Netherlands) solution of 100 μM, and were stratified at 4 °C for 3 d. After rinsing in deionized water three times, the stratified seeds were sown in planting holes (one seed per hole) of a hydroponic system (Fig. 1), with 0.7% Plant Agar (Fisher Scientific, Geel, Belgium), and rockwool cubes (Starter Plugs, Grodan Inc., Ontario, Canada). The two genotypes were evenly and randomly distributed in different rows (i.e., five rows for each genotype) within each tray. The sown trays were placed under the light treatments in a walk-in growth chamber. The ferti-gation method and the environment condition for growing the plants followed the way by Kong and Zheng (2020).

Fig. 1.

Side-view diagram of a hydroponic system used for growing Arabidopsis plants in this experiment.

cjps-2021-0018f1.tif

Light treatments and arrangement

Light treatments included: (1) R, a pure RL from 660 nm LED; (2) B, a pure BL from 455 nm LED; (3) BR, a impure BL from LED combination of 94% B and 6% R; and (4) BRF, another impure BL from LED combination of BR and 6 μmol·m−2·s−1 of FR (735 nm)(Fig. 2). Based on the light spectral distribution, the phytochrome photostationary state (PPS), also called phytochrome photoequilibrium (i.e., the ratio of active phytochrome to total phytochrome), was calculated for each of the four light treatments according to Sager et al. (1988). The calculated PPS values, indicators of phytochrome activity, were 0.89, 0.69, 0.60, and 0.50 for R, BR, BRF, and B, respectively. The light treatments were achieved by adjusting the intensities and spectra of a LX602C LED lighting system (Heliospectra AB, Gothenburg, Sweden) using System Assistant 2.0.1 (Heliospectra AB). In the chamber, the four light treatments were arranged to four divided compartments randomly. Opaque curtains were used to separate these compartments to avoid neighboring light pollution. For each light treatment, a photosynthetic photon flux density (PPFD) of around 100 μmol·m−2·s−1 was achieved at the plant canopy level. Light quality and intensity were set up and verified for the light treatments using a USB2000 + UV/VIS spectrometer (Ocean Optics, Inc., Dunedin, FL, USA).

Fig. 2.

The spectral distribution and PPS (phytochrome photostationary state) values of four light treatments delivered by light emitting diodes (LEDs). R = a pure red light with a peak at 660 nm; B = a pure blue light with a peak at 455 nm; BR = an impure blue light with a combination of 94% B and 6% R; and BRF = another impure blue light with a combination of BR and 6 μmol·m−2·s−1 of FR (735 nm). The numbers inside the figures are PPS values estimated according to Sager et al. (1988). [Colour online.]

cjps-2021-0018f2.tif

Biometric measurements

Once seed germination was over 50% for each genotype under each light treatment, the cumulative germination percentages were determined. After 18-d lighting, five plants from each genotype in each of light treatments (i.e., one plant from each row in each tray) were randomly selected for investigating plant morphology. The observed plant traits included main stem length, hypocotyl length, rosette leaf number, total leaf number, flowering index, and leaf morphology (size and color). The values of flowering index (ranging from 0–3) were defined as the same as our previous study (Kong and Zheng 2020). Leaf size and color were observed following the method by Kong et al. (2019b) and Karcher and Richardson (2003).

Statistical analysis

DPS 7.05 Software (Refine Information Tech. Co., Hangzhou, China), a data processing System, was used for the data analysis. In this experiment, the chamber environment conditions were uniform except for light treatments, and five rows of plants in growing trays were randomly distributed to each combination of light treatments × Arabidopsis genotypes. In this case, the experimental arrangement can be considered as a completely random design with two factors and five replicates. Two-way ANOVA was used to determine the effects of each factor (i.e., light treatment, or Arabidopsis genotype), and their interaction. For each plant trait, means separation for different treatments were determined using Duncan’s new multiple range test (P ≤ 0.05).

Results

Cumulative germination percentage was not different among the different treatments ( Supplementary Fig. S1 (cjps-2021-0018suppla.doc)11). Under the light treatments, main stem length differed in response pattern between phytochrome mutant and wild type plants (Fig. 3A). For wild type plants, B and BRF promoted main stem elongation relative to R or BR, and BR showed an inhibitory effect similar to R, but BRF vs. B had a greater promotion effect. For the phytochrome mutant, plants under B and R showed a similar height, but were shorter than those under BR and BRF, and plants were taller under BR than BRF. Phytochrome mutant had reduced main stem length under B or BRF, but increased main stem length under BR compared with wild type. It suggested that the absence of phytochromes attenuated the enhancement effect of B or BRF and removed the inhibition effect of BR on main stem elongation.

Fig. 3.

Stem elongation of wild-type Arabidopsis and its phytochrome-deficient mutant growing under different light spectra. WT = wild type; P = quintuple phytochrome (phyA phyB phyC phyD phyE) mutant. For the four light treatments, R = a pure red light from 660 nm LED; B = a pure blue light from 455 nm LED; BR = an impure blue light from LED combination of 94% B and 6% R; and BRF = another impure blue light from LED combination of BR and 6 μmol·m−2·s−1 of FR (735 nm). Data are presented as means ± SE (n = 5). The symbols inside the chart, i.e., L, G and L × G denote light treatment, plant genotype, and their interaction, respectively. Behind the symbols, ns, *, **, or *** indicate no significance or significance at a level of 0.05, 0.01, or 0.001, respectively, for the effect of treatment on the plant trait. Different letters on the data indicate significant difference (Duncan’s new multiple range test, P ≤ 0.05). [Colour online.]

cjps-2021-0018f3.tif

For hypocotyl length, the light response pattern of phytochrome mutant was similar to that of wild type; B, BR, and BRF reduced this trait compared with R, while the three BL treatments were not different from each other (Fig. 3B). The different response in hypocotyl from main stem suggests that BL-mediated elongation growth differed during the early and late growth stages. Under R, phytochrome mutant showed greater hypocotyl length than wild type plants. Hypocotyl was longer under BR for phytochrome mutant than wild type. It suggested that in this case during early growth stage, BL was more effective to inhibit elongation growth than RL, showing an inhibition effect independent of phytochrome.

For total leaf number, the light response pattern of phytochrome mutant was different from that of wild type. B, BR, and BRF, compared with R, did not change total leaf number for wild type, but increased this trait for phytochrome mutant (Fig. 4A). Under R, despite promoting hypocotyl elongation during early growth stage, the quintuple phytochrome mutant was not able to develop beyond some rudimentary leaves at the late stage. This might contribute to the different response of total leaf number between wild and mutant plants.

Fig. 4.

Leaf number and plant flowering of wild-type Arabidopsis and its phytochrome-deficient mutant growing under different light spectra. WT = wild type; P = quintuple phytochrome (phyA phyB phyC phyD phyE) mutant. For the four light treatments, R = a pure red light from 660 nm LED; B = a pure blue light from 455 nm LED; BR = an impure blue light from LED combination of 94% B and 6% R; and BRF = another impure blue light from LED combination of BR and 6 μmol·m−2·s−1 of FR (735 nm). Data are presented as means ± SE (n = 5). The symbols inside the chart, i.e., L, G and L × G denote light treatment, plant genotype, and their interaction, respectively. Behind the symbols, ns, *, **, or *** indicate no significance or significance at a level of 0.05, 0.01, or 0.001, respectively, for the effect of treatment on the plant trait. Different letters on the data indicate significant difference (Duncan’s new multiple range test, P ≤ 0.05). [Colour online.]

cjps-2021-0018f4.tif

For rosette leaf number, its light response pattern was similar to total leaf number for the phytochrome mutant, but different from total leaf number for wild type (Fig. 4B). All the BLs (i.e., B, BR, and BRF), relative to R, increased rosette leaf number in phytochrome mutant, but reduced this trait in wild type. For BL, rosette leaves under BR were increased compared with B and BRF for wild-type plants, but were reduced compared with B for phytochrome-deficient mutants. Also, under BR, the phytochrome mutant had less rosette leaves than wild type.

For flowering index, its light response pattern was generally similar to the response of main stem length, but different between wild type and phytochrome mutant (Fig. 4C). In wild type, flowering index was increased under B and BRF, compared with R or BR, but was not different between BR and R, or between BRF and B. In phytochrome mutant, flowering index was increased under B, BR, and BRF compared with R, and the promotion effect was greater for BR than B and BRF, and was similar for B and BRF. Under BR, phytochrome mutant showed a much greater flowering index than wild type. It suggested that for wild Arabidopsis, low-PPS BL (i.e., B or BRF) promoted flowering, but high-PPS BL (i.e., BR) inhibited flowering. However, in the absence of phytochromes, BR lost flowering inhibition effect, and promoted flowering to a greater degree than B or BRF.

For petiole length, the light response pattern of wild type was different from that of phytochrome mutant (Fig. 5A). In wild type, BR and BRF reduced petiole length compared with R, but for the phytochrome mutant, BR vs. R increased this trait, and there was no difference between BRF and R. Compared with wild type, phytochrome mutant had a longer petiole under BR, but a shorter petiole under R. This indicated that in the absence of phytochromes BR vs. R lost the inhibition effect and showed a promotion effect on petiole elongation.

Fig. 5.

Leaf size and color of wild-type Arabidopsis and its phytochrome-deficient mutant growing under different light spectra. WT = wild-type; P = quintuple phytochrome (phyA phyB phyC phyD phyE) mutant. For the four light treatments, R = a pure red light from 660 nm LED; B = a pure blue light from 455 nm LED; BR = an impure blue light from LED combination of 94% B and 6% R; and BRF = another impure blue light from LED combination of BR and 6 μmol·m−2·s−1 of FR (735 nm). Data are presented as means ± SE (n = 5). The symbols inside the chart, i.e., L, G and L × G denote light treatment, plant genotype, and their interaction, respectively. Behind the symbols, ns, *, **, or *** indicate no significance or significance at a level of 0.05, 0.01, or 0.001, respectively, for the effect of treatment on the plant trait. Different letters on the data indicate significant difference (Duncan’s new multiple range test, P ≤ 0.05). [Colour online.]

cjps-2021-0018f5.tif

For blade size (i.e., maximum blade length and width) and leaf area, the light response pattern of wild type was different from that of phytochrome mutant (Figs. 5B5D). In wild type, BRF, compared with R or B, reduced blade size and leaf area, but B or BR had similar effects as R on these traits. In the phytochrome mutant, the blade size and leaf area were increased under B, BR, or BRF relative to R, and were reduced under BRF vs. BR. Under both B and R, phytochrome mutant, compared with wild type, had reduced blade size and leaf area. This indicates that the absence of phytochrome inhibited the leaf expansion under B or R.

For leaf color, the light response pattern of wild type was different from that of phytochrome mutant (Fig. 5E). In wild type, leaf hue angle was not different among the light treatments. In phytochrome mutant, leaf hue angle was increased under BR, compared with R or BRF, but there was no difference among R, B and BRF. Under R, B or BRF, phytochrome mutant, compared wild type, had decreased leaf hue angle. This indicates that in the absence of phytochromes, leaves under R, B or BRF reduced greenness.

Discussion

BL’s effect on stem elongation of mature Arabidopsis plants is related to phytochrome activity

Similar to bedding plants (Kong et al. 2018), compared with R, wild Arabidopsis plants, showed longer main stem under BL with lower PPS (i.e., B or BRF), rather than BL with higher PPS (i.e., BR), suggesting that BL’s effect is related to phytochrome activity. Also, in wild type Arabidopsis, BRF promoted stem elongation to a larger degree compared with B, possibly due to an additive promotion effect of FR (Kusuma and Bugbee 2021). This differed from bedding plants where BRF showed a similar promotion effect as B (Kong et al. 2018). The mechanism underlying the different responses among plant species needs further study. Unlike main stem, hypocotyl elongation responses to BLs did not vary with different phytochrome activity, indicating by the different PPS values of BLs. For hypocotyl elongation, all the BL treatments (B, BR, and BRF) showed similar inhibitory effects compared with R. This also indicates that in mature plants only, our first hypothesis that wild Arabidopsis under BL show a stem elongation response pattern similar to bedding plants cannot be rejected. Similar inhibitory effects of BL vs. RL on hypocotyl elongation has been found in a previous study on Arabidopsis (Ahmad and Cashmore 1997). However, differing from our current study, the BL’s inhibition effect was strengthened when followed by a RL pulse and weakened when followed by a FR pulse, suggesting the contribution of phytochrome activity to BL-mediated hypocotyl elongation. The inconsistency of the results may be partly explained by the different light intensity employed in the two studies: a much lower BL intensity was used in the previous study (≈ 30 μmol·m−2·s−1) than in our present study (≈ 100 μmol·m−2·s−1). At least for some species, inhibitory effect on elongation by either pure or impure BL strengths with light intensity increasing (Cope and Bugbee 2013; Johnson et al. 2019). Therefore, in the present study, regardless of phytochrome activity, BL at a PPFD of 100 μmol·m−2·s−1 might be strong enough to inhibit hypocotyl elongation during de-etiolation stage, rather than stem elongation in mature plants for Arabidopsis. The stronger effect of light intensity than phytochrome activity on BL-mediated elongation may also help explain shorter plant stems developed under BL vs. RL even in some recent studies using LED lighting (Chen et al. 2014; Izzo et al. 2020; Vitale et al. 2020).

Although the PPS values can be easily used to indicate the phytochrome activities induced by different BL treatments, they may not reflect the real situation, and thus may lack accuracy. The reason lies in that the PPS value is calculated phytochrome photoequilibrium according to absorption of light spectrum, which was measured in the solution with isolated and purified phytochrome, rather than in plant leaves (Sager et al. 1988). Masking pigments (predominantly chlorophyll), and leaf structure can alter intracellular light regimes around phytochrome (Gardner and Graceffo 1982). Studying the difference between phytochrome mutants and wild type is another way to explore the involvement of phytochrome in BL-mediated elongation growth. However, many early studies on phyA phyB mutants cannot exclude the involvement of other residual phytochrome species (Strasser et al. 2010). In this case, quintuple phytochrome mutant, which is deficient of all the currently known phytochrome species, may provide a new plant material to study the mechanism of BL’s action on stem elongation.

Phytochrome play an active role in BL-mediated stem elongation of mature Arabidopsis

The pattern of main stem length response to the BL treatments was totally different between the phytochrome mutant and the wild Arabidopsis in the present study. For plants under the light treatments following an order of, R, B, BR, and BRF, main stem was short-tall-short-tall for wild type, but was short-short-tall-short for phytochrome mutant. The different response between the wild type and phytochrome mutant suggests that phytochrome is actively involved in the BL-mediated main stem elongation. Recent studies on Arabidopsis indicates that transcriptional changes in response to BL can be coordinately regulated by a cross talk at least between cryptochrome and phytochrome due to some of the shared signaling pathways (Liu et al. 2016; Pedmale et al. 2016; Mishra and Khurana 2017; Su et al. 2017; Yang et al. 2017). Possibly, phytochrome activity can modify the function of cryptochrome, the BL receptor, on main stem elongation (Liu et al. 2016).

Obviously, the above difference in main stem length between wild type and phytochrome mutant plants resulted from their different responses to each of the three BL treatments. Under BR (i.e., BL with a higher PPS value), main stem was the tallest for phytochrome mutant, but was the shortest for wild type among the light treatments. The reversal effect of BR on elongation in the presence or absence of phytochrome indicated that active phytochrome played an important role in the inhibitory effect of BR on stem elongation for wild Arabidopsis. Under B or BRF (i.e., BL with lower PPS values), phytochrome mutant reduced main stem length compared with wild type. In the absence of phytochrome, the promotion effect on main stem elongation was eliminated under B and reduced under BRF relative to R. The reduced elongation response in phytochrome mutant indicated that low-activity phytochrome might contribute partly to increased main stem elongation under BL associated with low PPS for wild types. Possibly, some other photoreceptors (e.g., phototropins), in addition to phytochromes, were also partly involved in the BL-promoted elongation (Kong and Zheng 2020).

Differing from main stem length, hypocotyl length was reduced under B, BR, and BRF relative to R for both phytochrome mutant and wild type, showing inhibitory hypocotyl elongation response to the BLs for the two Arabidopsis genotypes. It appears that phytochrome is not required for cryptochrome to inhibit hypocotyl elongation under BL in some cases (Strasser et al. 2010). It is well known that hypocotyl elongation occurs only at early growth stage, but main stem elongation lasts until late growth stage. Possibly, the involvement of phytochromes in the BL-mediated elongation was less significant during early vs. late growth stage for Arabidopsis under the conditions (e.g., ≈ 100 μmol·m−2·s−1) in the present study. Consequently, the second hypothesis that quintuple phytochrome mutants differ from wild-type plants in their elongation responses to light treatments cannot be rejected only at late growth stage. However, the BL’s inhibition effect on hypocotyl length was greater for phytochrome mutant than wild type. This was mainly due to the failed inhibition of hypocotyl elongation growth by R for the phytochrome mutant rather than wild type. Similar stretching hypocotyl response to RL has been found in phyA phyB double mutant of Arabidopsis (Reed et al. 1994), phyA phyB phyC triple mutant of rice (Takano et al. 2009), and quintuple phytochrome mutant of Arabidopsis (Strasser et al. 2010).

BL-promoted elongation growth of mature Arabidopsis plants is a shade-avoidance response partly mediated by phytochrome

In the current study, for the wild type plants, B or BRF, compared with R or BR, not only increased main stem length, but also promoted flowering and reduced leaf size (including leaf area, and maximum blade length and width), showing typical shade-avoidance responses (Casal 2012). Similar shade-avoidance responses have been also observed under B or BRF in our recent study on bedding plants (Kong et al. 2018). It is worthwhile to note that, for wild type Arabidopsis, plants under B or BRF relative to R or BR did not show shade-avoidance responses in some traits such as hypocotyl length, petiole length, and leaf color. It appeared that for the same plant, different plant traits had varied sensitivity in shade-avoidance response to BL with low PPS. This was supported by our previous studies on other plant species (Kong et al. 2018; Kong et al. 2019b). Hypocotyl length and leaf morphology showed a lower sensitivity in shade-avoidance response to BL with low PPS than main stem length and flowering index. This possibly resulted partly from varied threshold levels for BL to induce the shade-avoidance response at different stages or in different cells (Mishra and Khurana 2017).

Differing from wild type plants, phytochrome-deficient plants showed some antagonized shade-avoidance responses under B or BRF rather than BR or R. For phytochrome mutant, B or BRF increased leaf size relative to R, delayed flowering, reduced petiole length and main stem length relative to BR. It appears that the absence of phytochromes can prevent the shade-avoidance responses induced in wild types under BL with low PPS (i.e., B or BRF), suggesting a role played by phytochromes in the responses. Obviously, the third hypothesis that quintuple phytochrome mutant can change the shade-avoidance response induced in wild types under B or BRF relative to BR or R cannot be rejected. However, even in absence of phytochromes, some shade-avoidance responses (e.g., reduced leaf size and greenness) were still found under BRF vs. BR. This suggests that some other photoreceptors (e.g., phototropins), in addition to phytochrome, might be partly involved in the shade-avoidance response of wild Arabidopsis induced by BL with low PPS (Kong and Zheng 2020).

Conclusion

Overall, for wild type Arabidopsis plants under 24-h LED lighting at a PPFD of ≈100 μmol·m−2·s−1, BL with low PPS (i.e., B or BRF), relative to R, promoted main stem elongation, but BL with high PPS (i.e., BR) showed a similar inhibition effect as R. The absence of phytochrome reduced and even eliminated the promotion effect of B and BRF, and reversed BR effect from inhibition to promotion. However, regardless of PPS values, all the BLs (i.e., B, BR and BRF) relative to R reduced hypocotyl length in both wild types and phytochrome mutants. This suggests that it is in mature Arabidopsis plants that BL’s effect on stem elongation is related to phytochrome activity, and phytochrome is actively involved in BL-mediated stem elongation. Along with enhanced main stem elongation, B and BRF, compared with R or BR, also induced earlier flowering and reduced leaf size in wild type plants, showing typical shade-avoidance responses. In the absence of phytochrome, the above shade-avoidance responses were prevented under B or BRF, and induced under BR. Therefore, phytochrome contributes to BL-mediated stem elongation and the associated shade-avoidance responses in mature Arabidopsis plants.

Notes

1 Supplementary data are available with the article at  https://doi.org/10.1139/cjps-2021-0018.

Acknowledgements

This study was supported by Natural Sciences and Engineering Research Council of Canada. The LED lighting fixtures used for this study were provided by Heliospectra AB (Gothenburg, Sweden). We are grateful to Dr. Pablo D. Cerdána and Dr. Maximiliano Sánchez-Lamasa for their donation of quintuple phytochrome mutant seeds, and instruction of seed germination promotion. We thank Dr. Yongmei Bi for her donation of wild-type Arabidopsis and instruction on cultivation technique. Thanks also go to Katherine Schiestel and Dave Llewellyn for their excellent technical supports during the trial and Devdutt Kamath for his help with editing the manuscript.

References

1.

Ahmad, M., and Cashmore, A.R. 1997. The blue-light receptor cryptochrome 1 shows functional dependence on phytochrome A or phytochrome B in Arabidopsis thaliana. Plant J. 11: 421–427. https://doi.org/10.1046/j.1365-313x.1997.11030421.x. pmid:9107032Google Scholar

2.

Appelgren, M. 1991. Effects of light quality on stem elongation of Pelargonium in vitro. Sci. Hort. 45: 345–351. https://doi.org/10.1016/0304-4238(91)90081-9Google Scholar

3.

Bergstrand, K.J., Asp, H., and Schüssler, H.K. 2014. Development and acclimatisation of horticultural plants subjected to narrow-band lighting. Eur. J. Hortic. Sci. 79: 45–51. Google Scholar

4.

Brown, C.S., Schuerger, A.C., and Sager, J.C. 1995. Growth and photomorphogenesis of pepper plants under red light-emitting diodes with supplemental blue or far-red lighting. J. Am. Soc. Hortic. Sci. 120: 808–813. https://doi.org/10.21273/jashs.120.5.808. pmid:11540133Google Scholar

5.

Casal, J.J. 2012. Shade avoidance. The Arabidopsis book. 10: e0157. https://doi.org/10.1199/tab.0157. pmid:22582029Google Scholar

6.

Chen, X., Guo, W., Xue, X., Wang, L., and Qiao, X. 2014. Growth and quality responses of ‘Green Oak Leaf’ lettuce as affected by monochromic or mixed radiation provided by fluorescent lamp (FL) and light-emitting diode (LED). Sci. Horti. 172: 168–175. https://doi.org/10.1016/j.scienta.2014.04.009Google Scholar

7.

Chory, J., Peto, C.A., Ashbaugh, M., Saganich, R., Pratt, L., and Ausubel, F. 1989. Different roles for phytochrome in etiolated and green plants deduced from characterization of Arabidopsis thaliana mutants. Plant Cell. 1: 867–880. https://doi.org/10.2307/3868934. pmid:12359912Google Scholar

8.

Cope, K.R., and Bugbee, B. 2013. Spectral effects of three types of white light-emitting diodes on plant growth and development: absolute versus relative amounts of blue light. HortScience. 48: 504–509. https://doi.org/10.21273/hortsci.48.4.504Google Scholar

9.

Cosgrove, D.J. 1981. Rapid suppression of growth by blue light occurrence, time course, and general characteristics. Plant Physiol. 67: 584–590. https://doi.org/10.1104/pp.67.3.584. pmid:16661718Google Scholar

10.

Franklin, K.A., and Quail, P.H. 2010. Phytochrome functions in Arabidopsis development. J. Exp. Bot. 61: 11–24. https://doi.org/10.1093/jxb/erp304. pmid:19815685Google Scholar

11.

Fukuda, N., Ajima, C., Yukawa, T., and Olsen, J.E. 2016. Antagonistic action of blue and red light on shoot elongation in petunia depends on gibberellin, but the effects on flowering are not generally linked to gibberellin. Environ. Exp. Bot. 121: 102–111. https://doi.org/10.1016/j.envexpbot.2015.06.014Google Scholar

12.

Gardner, G., and Graceffo, M.A. 1982. The use of a computerized spectroradiometer to predict phytochrome photoequilibria under polychromatic irradiation. Photochem. Photobiol. 36: 349–354. https://doi.org/10.1111/j.1751-1097.1982.tb04385.xGoogle Scholar

13.

Hata, N., Hayashi, Y., Ono, E., Satake, H., Kobayashi, A., Muranaka, T., and Okazawa, A. 2013. Differences in plant growth and leaf sesamin content of the lignan-rich sesame variety ‘Gomazou’ under continuous light of different wavelengths. Plant Biotechnol. 30: 1–8. https://doi.org/10.5511/plantbiotechnology.12.1021aGoogle Scholar

14.

Heo, J., Lee, C., Chakrabarty, D., and Paek, K. 2002. Growth responses of marigold and salvia bedding plants as affected by monochromic or mixture radiation provided by a light-emitting diode (LED). Plant Growth Regul. 38: 225–230. https://doi.org/10.1023/a:1021523832488Google Scholar

15.

Hernandez, R., and Kubota, C. 2016. Physiological responses of cucumber seedlings under different blue and red photon flux ratios using LEDs. Environ. Exp. Bot. 121: 66–74. https://doi.org/10.1016/j.envexpbot.2015.1004.1001Google Scholar

16.

Hirai, T., Amaki, W., and Watanabe, H. 2006. Action of blue or red monochromatic light on stem internodal growth depends on plant species. Acta Hort. 711: 345–350. https://doi.org/10.17660/actahortic.2006.711.47Google Scholar

17.

Hoenecke, M.E., Bula, R.J., and Tibbitts, T.W. 1992. Importance of ‘blue’ photon levels for lettuce seedlings grown under red-light-emitting diodes. HortScience, 27: 427–430. https://doi.org/10.21273/hortsci.27.5.427. pmid:11537611Google Scholar

18.

Izzo, L.G., Mele, B.H., Vitale, L., Vitale, E., and Arena, C. 2020. The role of monochromatic red and blue light in tomato early photomorphogenesis and photosynthetic traits. Environ. Exp. Bot. 179: 104195. https://doi.org/10.1016/j.envexpbot.2020.104195Google Scholar

19.

Johnson, R.E., Kong, Y., and Zheng, Y. 2019. Elongation growth mediated by blue light varies with light intensities and plant species: A comparison with red light in arugula and mustard seedlings. Environ. Exp. Bot. 169: 103898. https://doi.org/10.1016/j.envexpbot.2019.103898Google Scholar

20.

Karcher, D.E., and Richardson, M.D. 2003. Quantifying turfgrass color using digital image analysis. Crop Sci. 43: 943–951. https://doi.org/10.2135/cropsci2003.9430Google Scholar

21.

Kim, E.Y., Park, S.A., Park, B.J., Lee, Y., and Oh, M.M. 2014. Growth and antioxidant phenolic compounds in cherry tomato seedlings grown under monochromatic light-emitting diodes. Hort. Environ. Biotechnol. 55: 506–513. https://doi.org/10.1007/s13580-014-0121-7Google Scholar

22.

Kong, Y., and Zheng, Y. 2020. Phototropin is partly involved in blue-light-mediated stem elongation, flower initiation, and leaf expansion: a comparison of phenotypic responses between wild Arabidopsis and its phototropin mutants. Environ. Exp. Bot. 171: 103967. https://doi.org/10.1016/j.envexpbot.2019.103967Google Scholar

23.

Kong, Y., Wang, S., Chen, J., Chen, Q., and Yao, Y. 2012. Effect of supplemental lighting with red and blue light on the characters of container-growing seedlings of muskmelon. Acta Hort. 944: 141–146. https://doi.org/10.17660/actahortic.2012.944.18Google Scholar

24.

Kong, Y., Stasiak, M., Dixon, M.A., and Zheng, Y. 2018. Blue light associated with low phytochrome activity can promote elongation growth as shade-avoidance response: a comparison with red light in four bedding plant species. Environ. Exp. Bot. 155: 345–359. https://doi.org/10.1016/j.envexpbot.2018.07.021Google Scholar

25.

Kong, Y., Kamath, D., and Zheng, Y. 2019a. Blue versus red light can promote elongation growth independent of photoperiod: a study in four Brassica microgreens species. HortScience, 54: 1955–1961. https://doi.org/10.21273/hortsci14286-19Google Scholar

26.

Kong, Y., Schiestel, K., and Zheng, Y. 2019b. Pure blue light effects on growth and morphology are slightly changed by adding low-level UVA or far-red light: a comparison with red light in four microgreen species. Environ. Exp. Bot. 157: 58–68. https://doi.org/10.1016/j.envexpbot.2018.09.024Google Scholar

27.

Kong, Y., Schiestel, K., and Zheng, Y. 2020. Maximum elongation promoted as a shade-avoidance response by blue light is related to deactivated phytochrome: a comparison with red light in four microgreen species. Can. J. Plant Sci. 100: 314–326. https://doi.org/10.1139/cjps-2019-0082Google Scholar

28.

Koornneef, M., Rolff, E., and Spruit, C.J.P. 1980. Genetic control of light-inhibited hypocotyl elongation in Arabidopsis thaliana (L.) Heynh. Zeitschrift für Pflanzenphysiologie, 100: 147–160. https://doi.org/10.1016/s0044-328x(80)80208-xGoogle Scholar

29.

Kusuma, P., and Bugbee, B. 2021. Far-red fraction: An improved metric for characterizing phytochrome effects on morphology. J. Am. Soc. Hortic. Sci. 146: 3–13. https://doi.org/10.21273/jashs05002-20Google Scholar

30.

Legris, M., Ince, Y.Ç., and Fankhauser, C. 2019. Molecular mechanisms underlying phytochrome-controlled morphogenesis in plants. Nat. Commun. 10: 1–15. https://doi.org/10.1038/s41467-019-13045-0Google Scholar

31.

Liu, B., Yang, Z., Gomez, A., Liu, B., Lin, C., and Oka, Y. 2016. Signaling mechanisms of plant cryptochromes in Arabidopsis thaliana. J. Plant Res. 129: 137–148. https://doi.org/10.1007/s10265-015-0782-z. pmid:26810763Google Scholar

32.

Mishra, S., and Khurana, J.P. 2017. Emerging roles and new paradigms in signaling mechanisms of plant cryptochromes. Crit. Rev. Plant Sci. 36: 89–115. https://doi.org/10.1080/07352689.2017. 1348725Google Scholar

33.

Mizuno, T., Amaki, W., and Watanabe, H. 2011. Effects of monochromatic light irradiation by LED on the growth and anthocyanin contents in leaves of cabbage seedlings. Acta Hort. 907: 179–184. https://doi.org/10.17660/actahortic.2011.907.25Google Scholar

34.

Pedmale, U.V., Huang, S.C., Zander, M., Cole, B.J., Hetzel, J., Ljung, K., et al. 2016. Cryptochromes interact directly with PIFs to control plant growth in limiting blue light. Cell, 164: 233–245. https://doi.org/10.1016/j.cell.2015.12.018. pmid:26724867Google Scholar

35.

Reed, J.W., Nagatani, A., Elich, T.D., Fagan, M., and Chory, J. 1994. Phytochrome A and phytochrome B have overlapping but distinct functions in Arabidopsis development. Plant Physiol. 104: 1139–1149. https://doi.org/10.1104/pp.104.4.1139. pmid:12232154Google Scholar

36.

Sager, J.C., Smith, W.O., Edwards, J.L., and Cyr, K.L. 1988. Photosynthetic efficiency and phytochrome photoequilibria determination using spectral data. Trans. ASAE. 31: 1882–1889. https://doi.org/10.13031/2013.30952Google Scholar

37.

Schwend, T., Prucker, D., and Mempel, H. 2015. Red light promotes compact growth of sunflowers. Eur. J. Hortic. Sci. 80: 56–61. https://doi.org/10.17660/ejhs.2015/80.2.2Google Scholar

38.

Strasser, B., Sanchez-Lamas, M., Yanovsky, M.J., Casal, J.J., and Cerdan, P.D. 2010. Arabidopsis thaliana life without phytochromes. Proc. Natl. Acad. Sci. 107: 4776–4781. https://doi.org/10.1073/pnas.0910446107. pmid:20176939Google Scholar

39.

Stutte, G.W. 2009. Light-emitting diodes for manipulating the phytochrome apparatus. HortScience, 44: 231–234. https://doi.org/10.21273/hortsci.44.2.231Google Scholar

40.

Su, J., Liu, B., Liao, J., Yang, Z., Lin, C., and Oka, Y. 2017. Coordination of cryptochrome and phytochrome signals in the regulation of plant light responses. Agronomy, 7: 25. https://doi.org/10.3390/agronomy7010025Google Scholar

41.

Takano, M., Inagaki, N., Xie, X., Kiyota, S., Baba-Kasai, A., Tanabata, T., and Shinomura, T. 2009. Phytochromes are the sole photoreceptors for perceiving red/far-red light in rice. Proc. Natl. Acad. Sci. 106: 14705–14710. https://doi.org/10.1073/pnas. 0907378106. pmid:19706555Google Scholar

42.

Vitale, L., Vitale, E., Guercia, G., Turano, M., and Arena, C. 2020. Effects of different light quality and biofertilizers on structural and physiological traits of spinach plants. Photosynthetica, 58: 932–943. https://doi.org/10.32615/ps.2020.039Google Scholar

43.

Wheeler, R.M., Mackowiak, C.L., and Sager, J.C. 1991. Soybean stem growth under high-pressure sodium with supplemental blue lighting. Agron. J. 83: 903–906. https://doi.org/10.2134/agronj1991.00021962008300050024x. pmid:11537676Google Scholar

44.

Yang, Z., Liu, B., Su, J., Liao, J., Lin, C., and Oka, Y. 2017. Cryptochromes orchestrate transcription regulation of diverse blue light responses in plants. Photochem. Photobiol. 93: 112–127. https://doi.org/10.1111/php.12663. pmid:27861972Google Scholar

45.

Young, J.C., Liscum, E., and Hangarter, R.P. 1992. Spectral-dependence of light-inhibited hypocotyl elongation in photomorphogenic mutants of Arabidopsis: evidence for a UV-A photosensor. Planta, 188: 106–114. https://doi.org/10.1007/bf01160719. pmid:24178206Google Scholar
© 2021 The Author(s).
Yun Kong and Youbin Zheng "Phytochrome contributes to blue-light-mediated stem elongation and flower initiation in mature Arabidopsis thaliana plants," Canadian Journal of Plant Science 102(2), 449-458, (13 September 2021). https://doi.org/10.1139/CJPS-2021-0018
Received: 18 January 2021; Accepted: 27 August 2021; Published: 13 September 2021
KEYWORDS
Arabidopsis thaliana
Arabidopsis thaliana
blue light
floraison
flowering time
hypocotyl length
Leaf size
Back to Top