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8 July 2014 Quick test for qualitative detection of glyphosate-resistant kochia (Kochia scoparia L. Schrad.)
Jessica Pratchler, Scott W. Shirriff, Hugh J. Beckie
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Abstract

Pratchler, J., Shirriff, S. W. and Beckie, H. J. 2014. Quick test for qualitative detection of glyphosate-resistant kochia (Kochia scoparia L. Schrad.). Can. J. Plant Sci. 94: 1413-1417. A growth chamber study was conducted to determine the feasibility of developing a quick, yet reliable assay to detect glyphosate-resistant (GR) kochia. Seeds of six kochia populations, three GR and three non-GR (susceptible), were placed on filter paper in dishes treated with varying doses of glyphosate. After 7-d incubation, seedlings with a true leaf (i.e., not cotyledons) at 25 mg L-1 glyphosate accurately distinguished GR from susceptible kochia seed lots. This bioassay can facilitate more timely confirmation of GR kochia, thereby aiding resistant weed management.

Jessica Pratchler, Scott W. Shirriff, and Hugh J. Beckie "Quick test for qualitative detection of glyphosate-resistant kochia (Kochia scoparia L. Schrad.)," Canadian Journal of Plant Science 94(8), 1413-1417, (8 July 2014). https://doi.org/10.1139/CJPS-2014-180
Received: 9 May 2014; Accepted: 1 July 2014; Published: 8 July 2014
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KEYWORDS
Assay
bioassay
diagnostic test
épreuve biologique
Glyphosate resistance
Herbicide resistance
Kochia scoparia L. Schrad.
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