Translator Disclaimer
13 April 2016 Functional analysis of the 1-aminocyclopropane-1- carboxylate deaminase gene of the biocontrol fungus Trichoderma asperellum ACCC30536
Fuli Zhang, Zhihua Liu, Mijiti Gulijimila, Yucheng Wang, Haijuan Fan, Zhiying Wang
Author Affiliations +
Abstract

1-aminocyclopropane-1-carboxylate (ACC) deaminase (ACCD) cleaves ACC, the immediate precursor of the ethylene, decreasing the level of ethylene and inhibition of plant growth resulted by environmental stresses. Here, TaACCD was cloned from the biocontrol agent Trichoderma asperellum ACCC30536. Its open reading frame was 1047 bp long encoding a 37 kD protein of 348 aa, and a pI of 5.77. Phylogenetic analysis demonstrated this protein to be closely related to ACCD from T. asperellum T203 (ACX94231). Transformation of Populus davidiana × P. bolleana with TaACCD, increased salinity tolerance of transgenic plants Pdb-ACCD3 and Pdb-ACCD5. Transgenic plants could survive at salinity of 200 mM NaCl, whereas untransformed control poplar Pdb-NT could withstand salinity to 150 mM NaCl. Transformed plants accumulated higher amounts of chlorophyll compared to Pdb-NT plants. Accumulation of reactive oxygen species (ROS) was regulated by TaACCD under salt stress, as shown from higher superoxide dismutase (SOD) and peroxidase (POD) activities, as well as NBT and DAB staining. Evans blue staining showed that TaACCD maintained membrane integrity in Populus under salt stress conditions. Additionally, TaACCD expression decreased ethylene content of transgenic plants compared to nontransgenic plants, but salt content in plant leaves didnt show obvious difference under same salt concentration. To the best of our knowledge, the current study is the first demonstration that the TaACCD gene from T. asperellum ACCC30536 can enhance tolerance of Populus to salt stress.

Fuli Zhang, Zhihua Liu, Mijiti Gulijimila, Yucheng Wang, Haijuan Fan, and Zhiying Wang "Functional analysis of the 1-aminocyclopropane-1- carboxylate deaminase gene of the biocontrol fungus Trichoderma asperellum ACCC30536," Canadian Journal of Plant Science 96(2), 265-275, (13 April 2016). https://doi.org/10.1139/cjps-2014-0265
Received: 19 July 2014; Accepted: 1 September 2015; Published: 13 April 2016
JOURNAL ARTICLE
11 PAGES


Share
SHARE
KEYWORDS
1-aminocyclopropane-1-carboxylate deaminase
1-aminocyclopropane-1-carboxylate désaminase
peuplier transgénique
salinity tolerance
tolérance au sel
transgenic poplar
Trichoderma asperellum
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top