Rapid determination of n-6 and n-3 fatty acid ratios in cereal grains and forages by 1H NMR spectroscopy

D. Prema; J. Jensen; J.L. Pilfold; T.D. Turner; K.K. Donkor; B. Cinel; J.S. Church

doi:10.1139/cjps-2015-0302

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D. Prema,¹ J. Jensen,¹ J.L. Pilfold,¹ T.D. Turner,¹ K.K. Donkor,¹ B. Cinel,^1,* J.S. Church²

¹aDepartment of Physical Sciences, Thompson Rivers University, Kamloops, BC V2C 0C8, Canada.
²bDepartment of Natural Resource Science, Thompson Rivers University, Kamloops, BC V2C 0C8, Canada.

^*Corresponding author: B. Cinel (email: bcinel@tru.ca).

Abstract

Herein we describe a new method to rapidly determine the n-6/n-3 fatty acid ratios in cereal grains and forages (hydroponically-grown cereal sprouts and haylage) using ¹H nuclear magnetic resonance (NMR) spectroscopy. This NMR method was found to be consistent with more established but time-consuming gas chromatographic methods (R = 0.994).

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Citation Download Citation

D. Prema, J. Jensen, J.L. Pilfold, T.D. Turner, K.K. Donkor, B. Cinel, and J.S. Church "Rapid determination of n-6 and n-3 fatty acid ratios in cereal grains and forages by ¹H NMR spectroscopy," Canadian Journal of Plant Science 96(5), 730-733, (18 April 2016). https://doi.org/10.1139/cjps-2015-0302

Received: 16 October 2015; Accepted: 13 March 2016; Published: 18 April 2016