Wild oat is the most economically detrimental weed species in the Canadian Prairies and effective herbicidal control options are limited due to widespread resistance to ACCase inhibitors, ALS inhibitors, and lipid biosynthesis inhibitors; therefore, evaluation of new herbicidal modes of action such as pyroxasulfone and sulfentrazone for control is critical. Two wild oat populations (HR08-210 and HR11-151) were first subjected to a discriminating dose screen to characterize resistance to ACCase and ALS inhibiting herbicides in comparison with a susceptible population (S1988). Dose-response experiments with triallate, pyroxasulfone, and sulfentrazone were then conducted to evaluate potential cross-resistance. Screening indicated both herbicide-resistant (HR) populations were resistant to ACCase- and ALS inhibiting herbicides, most likely due to enhanced metabolism and an ACCase mutation. HR08-210 and HR11-151 were resistant to triallate (resistance ratios of 2.53 and 3.39, respectively), but cross-resistance to pyroxasulfone (2.78) and sulfentrazone (2.0) was only observed in HR11-151. Results indicate previously selected resistance to ACCase and ALS inhibitors (enhanced metabolism and ACCase mutation) or triallate (enhanced endogenous gibberellins) could limit the utility of new herbicide modes of action for control of wild oat.