To explore candidate genes responsible for the blue color of Selaginella uncinata (Desv. ex Poir.) Spring leaves, we used transcriptome sequencing to compare differential gene expressions in blue and red S. uncinata leaves selected based on morphological observations and pigment content. In total, we obtained 30 119 unigenes with an average length of 1133 bp, and we identified 1442 differentially expressed genes (DEGs). Among the 1442 DEGs, 886 were upregulated and 556 were downregulated in blue leaf samples. After verification of expression by quantitative real-time polymerase chain reaction (qRT-PCR) analysis, seven key enzymes and their encoding genes were identified as being involved in the chlorophyll and flavonoid metabolism pathways. These genes included Mg-chelatase (CHLH), light-dependent NADPH-Pchlide oxidoreductase (LPOR), chlorophyllase (Chlase; CLH), hydroxycinnamoyltransferase (HCT), chalcone synthase (CHS), MYB, and flavonoid 3'-hydroxylase (F3'H), which may be involved in the blue leaf coloration of S. uncinata. Results from this study implied that the primary pathway of pigment metabolism in S. uncinata may be the chlorophyll metabolism pathway rather than the anthocyanin biosynthesis pathway. It is possible that chlorophyll b (Chl b) may be transformed into chlorophyll a (Chl a) by an alternative pathway in which it is converted to Chlorophyllide b by CLH, and then transformed to Chl a without the involvement of CBR or HCAR.
You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither BioOne nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the BioOne website.