Garden potato varieties grown on more than 1.5 ha are subject to variety registration to be sold as seed potato in Canada. Prerequisites for the registration of varieties include the pedigree and breeding history as well as a morphological description. For most varieties, no pedigree or morphological description is available, and furthermore, some groups are essentially derived from one another. Therefore, a review to determine the genetic and morphological similarities and uniqueness of some of the garden potato varieties was initiated. Seven garden potato varieties grown over the largest areas in Canada were genotyped using a set of nine microsatellite [simple sequence repeat (SSR)] markers, resulting in six distinct SSR profiles. These varieties were also grown out for morphological description, providing supplementary information for the national registration and their monitoring during the seed certification process. To further assess the genetic similarities of garden or heritage varieties, 84 potato varieties were genotyped, and the unique SSR profiles of 62 varieties and six SSR profiles shared among the other 22 varieties were added to the Canadian Food Inspection Agency potato variety reference SSR profile collection. Furthermore, a set of blue varieties were described morphologically to evaluate the nature of morphological characteristic variation of varieties sharing the same SSR profile. Although all 14 varieties grown show some morphological differences, all varieties within each group sharing an SSR profile also clustered together based on morphological characteristics.