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16 May 2018 Cloning and characterization of MdGST1 from red apple leaves
Xiaolei Han, Caixia Zhang, Yi Tian, Hera Gul, Peihua Cong, Liyi Zhang
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Glutathione S-transferase (GST) is involved in the downstream steps of the anthocyanin biosynthesis pathway in plants. However, the gene(s) encoding this enzyme have not been isolated from apple (Malus × domestica Borkh.) yet. We isolated a gene encoding GST from leaves of the red-fleshed apple variety ‘Royalty’ by full cDNA library sequencing and the 3′ rapid-amplification of cDNA ends method, and designated it MdGST1. In total, seven different MdGST1 transcripts were found. These had three different untranslated but identical protein-coding regions. Phylogenetic analysis showed that MdGST1 is a TT19-type GST, which is involved in anthocyanin transport. qRT-PCR analyses showed that the transcript level of MdGST1 was much higher in red leaves than in bagged or green leaves. When MdGST1 was introduced into a non-pigmented mutant of Arabidopsis, the transformants showed a visible purple phenotype in leaves and stems. Our results suggest that MdGST1 plays a role in anthocyanin biosynthesis. This information will help to improve the understanding of the mechanism of apple coloration.

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Xiaolei Han, Caixia Zhang, Yi Tian, Hera Gul, Peihua Cong, and Liyi Zhang "Cloning and characterization of MdGST1 from red apple leaves," Canadian Journal of Plant Science 98(5), 1150-1158, (16 May 2018).
Received: 1 November 2017; Accepted: 5 March 2018; Published: 16 May 2018

glutathione S-transferase
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