Sclerotinia sclerotiorum is a necrotrophic fungal pathogen with a very wide host range. Isolates of this pathogen are normally described as having fluffy and white mycelium; however, isolates of S. sclerotiorum with darkly-pigmented mycelium on potato dextrose agar medium have been identified in eastern New Mexico and western Texas from Valencia peanut fields. Mutant non-pigmented S. sclerotiorum isolates (SW) were created in an earlier study from wild-type pigmented isolates (SD) using melanin inhibitors. The SD isolates were pathogenic on Valencia peanut, whereas the SW isolates were not. The current study was conducted to further characterize the differences between SD and SW isolates in regards to metabolite production and utilization, including the effects of carbon sources and oxalic acid precursors on oxalic acid production and pathogenicity on Valencia peanut. Gas chromatography–mass spectrometry (GC/MS) metabolomics analysis revealed a down-regulation of several sugars and compounds within the citric acid cycle as well as oxalic acid for SW isolates of S. sclerotiorum. The addition of glucose to potato dextrose agar medium allowed for the production of oxalic acid and restored pathogenicity in SW isolates that were previously non-pathogenic on Valencia peanut. This study indicates that glucose alone plays a major role in oxalic acid production and pathogenicity of S. sclerotiorum.
You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither BioOne nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the BioOne website.