A rapid bioassay to screen soils for toxicity of residual petroleum hydrocarbons

John Ashworth; Ian Bullecer

doi:10.1139/CJSS2012-012

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1 November 2012 A rapid bioassay to screen soils for toxicity of residual petroleum hydrocarbons

John Ashworth, Ian Bullecer

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Canadian J. of Soil Science, 92(6):901-904 (2012). https://doi.org/10.1139/CJSS2012-012

Abstract

Ashworth, J. and Bullecer, I. 2012. A rapid bioassay to screen soils for toxicity of residual petroleum hydrocarbons. Can. J. Soil. Sci. 92: 901-904. The following 2-h procedure is proposed as a screen to indicate how soils contaminated with petroleum hydrocarbons (PHC) might perform in bioassays required for site remediation in Canada. Bio-available PHC in soil is extracted by aqueous cyclodextrin; a centrifuged extract portion is treated with amylase, then subjected to a standard Microtox^® bioassay. Extract toxicity is correlated to available PHC content.

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John Ashworth and Ian Bullecer "A rapid bioassay to screen soils for toxicity of residual petroleum hydrocarbons," Canadian Journal of Soil Science 92(6), 901-904, (1 November 2012). https://doi.org/10.1139/CJSS2012-012

Received: 31 January 2012; Accepted: 22 August 2012; Published: 1 November 2012

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