A survey of myxomycetes was conducted at three sites within Long Hunter State Park, Davidson County, Tennessee, from May 6 to September 30, 2006. Fruiting bodies that developed on samples of bark and plant debris placed in moist chamber cultures were used to supplement field collections. Results include 207 specimens representing 67 species of myxomycetes in 25 genera. Three species (Arcyria minuta, Licea cf. eleanorae, Licea cf. rugosa) appear to be new reports for the United States and a fourth species (Physarum auriscalpium) is a new report for Tennessee.