A survey to document the vascular flora of the Gopher Farm sandhill, Wayne County, Mississippi, was conducted from 2011 to 2015. As a part of the Chickasawhay Ranger District of the DeSoto National Forest, the Gopher Farm represents one of the most northerly sandhills in the Coastal Plain of Mississippi and one the largest publicly owned sandhills in the state. The survey resulted in 194 species, including 2 ferns, 3 gymnosperms, and 189 flowering plants. Of these, 45 are endemic to the Atlantic and Gulf Coastal Plain of the United States, and 6 are listed as rare species by the Mississippi Natural Heritage Program. The collection of Morella inodora represents the most northerly collection of that species in Mississippi.