Use of surface-enhanced laser desorption ionization–time-of-flight to identify heat shock protein 70 isoforms in closely related species of the virilis group of Drosophila

Olga G. Zatsepina; Alexander A. Karavanov; David G. Garbuz; Victoria Shilova; Peter Tornatore; Michael B. Evgen'ev

doi:10.1379/CSC-71.1

1 March 2005 Use of surface-enhanced laser desorption ionization–time-of-flight to identify heat shock protein 70 isoforms in closely related species of the virilis group of Drosophila

Olga G. Zatsepina, Alexander A. Karavanov, David G. Garbuz, Victoria Shilova, Peter Tornatore, Michael B. Evgen'ev

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Cell Stress & Chaperones, 10(1):12-16 (2005). https://doi.org/10.1379/CSC-71.1

Abstract

The 70-kDa heat shock protein (Hsp) family in all Drosophila species includes 2 environmentally inducible family members, Hsp70 and Hsp68. Two-dimensional gel electrophoresis revealed an unusual pattern of heat shock– inducible proteins in the species of the virilis group. Trypsin fingerprinting and microsequencing of tryptic peptides using ProteinChip Array technology identified the major isoelectric variants of Hsp70 family, including Hsp68 isoforms that differ in both molecular mass and isoelectric point from those in Drosophila melanogaster. The peculiar electrophoretic mobility is consistent with the deduced amino acid sequence of corresponding hsp genes from the species of the virilis group.

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Olga G. Zatsepina, Alexander A. Karavanov, David G. Garbuz, Victoria Shilova, Peter Tornatore, and Michael B. Evgen'ev "Use of surface-enhanced laser desorption ionization–time-of-flight to identify heat shock protein 70 isoforms in closely related species of the virilis group of Drosophila," Cell Stress & Chaperones 10(1), 12-16, (1 March 2005). https://doi.org/10.1379/CSC-71.1

Received: 16 July 2004; Accepted: 1 October 2004; Published: 1 March 2005

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