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1 June 2007 Identification of the PP2A-interacting region of heat shock transcription factor 2
Hongyan Xing, Yiling Hong, Kevin D. Sarge
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Previous work in our laboratory demonstrated the existence of an association between heat shock transcription factor 2 (HSF2) and the serine/threonine phosphatase 2A, which is mediated by interaction between HSF2 and the A subunit (also called PR65) of this protein phosphatase. In light of the importance of HSF2-PP2A association for HSF2 cellular function, in this study, we have sought to dissect the sequences within HSF2 that are important for interaction with the A subunit of PP2A. The results of these experiments indicate that the HSF2 region comprising amino acids 343–363 is important for A subunit interaction. This region includes part of the C-terminal leucine zipper motif of HSF2 called heptad repeat C (HR-C). The results of transfection/immunoprecipitation experiments also show that deletion of the 6 amino acids from 343 to 348 from HSF2 (HSF2 (Δ343–348)), is sufficient to prevent HSF2 from interacting with PP2A. These data provide insight into a new functional domain of HSF2, the PP2A A subunit-interacting region.

Hongyan Xing, Yiling Hong, and Kevin D. Sarge "Identification of the PP2A-interacting region of heat shock transcription factor 2," Cell Stress & Chaperones 12(2), 192-197, (1 June 2007).
Received: 9 November 2006; Accepted: 1 March 2007; Published: 1 June 2007

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