How to translate text using browser tools
1 August 2007 HSF2 binds to the Hsp90, Hsp27, and c-Fos promoters constitutively and modulates their expression
Donald C. Wilkerson, Hollie S. Skaggs, Kevin D. Sarge
Author Affiliations +

Although the vast majority of genomic DNA is tightly compacted during mitosis, the promoter regions of a number of genes remain in a less compacted state throughout this stage of the cell cycle. The decreased compaction of these promoter regions, which is referred to as gene bookmarking, is thought to be important for the ability of cells to express these genes during the following interphase. Previously, we reported a role for the DNA-binding protein heat shock factor (HSF2) in bookmarking the stress-inducible 70 000-Da heat shock protein (hsp70) gene. In this report, we have extended those studies and found that during mitosis, HSF2 is bound to the HSE promoter elements of other heat shock genes, including hsp90 and hsp27, as well as the proto-oncogene c-fos. The presence of HSF2 is important for expression of these genes because blocking HSF2 levels by RNA interference techniques leads to decreased levels of these proteins. These results suggest that HSF2 is important for constitutive as well as stress-inducible expression of HSE-containing genes.

Donald C. Wilkerson, Hollie S. Skaggs, and Kevin D. Sarge "HSF2 binds to the Hsp90, Hsp27, and c-Fos promoters constitutively and modulates their expression," Cell Stress & Chaperones 12(3), 283-290, (1 August 2007).
Received: 9 November 2006; Accepted: 1 April 2007; Published: 1 August 2007

This article is only available to subscribers.
It is not available for individual sale.

Get copyright permission
Back to Top