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1 October 2003 Molecular characterization of genes encoding cytosolic Hsp70s in the zygomycete fungus Rhizopus nigricans
Boštjan Černila, Bronislava Črešnar, Katja Breskvar
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Previous studies have shown that some stressors, including steroid hormones 21-OH progesterone and testosterone, stimulate the accumulation of heat shock protein 70 (hsp70) messenger ribonucleic acid (mRNA) population in the zygomycete filamentous fungus Rhizopus nigricans. In this study we report the cloning of 3 R nigricans hsp70 genes (Rnhsp70-1, Rnhsp70-2, and Rnhsp70-3) encoding cytosolic Hsp70s. With a Southern blot experiment under high stringency conditions we did not detect any additional highly homologous copies of the cytosolic hsp70 genes in the R nigricans genome. Sequence analyses showed that all 3 genes contain introns within the open reading frame. The dynamics of the R nigricans molecular response to progesterone, 21-OH progesterone, and testosterone, as well as to heat shock, copper ions, hydrogen peroxide, and ethanol was studied by temporal analysis of Rnhsp70-1 and Rnhsp70-2 mRNA accumulation. Northern blot experiments revealed that the Rnhsp70-2 transcript level is not affected by testosterone, whereas mRNA levels of both genes are rapidly increased with all the other stressors studied. Moreover, the decrease of transcript levels is notably delayed in ethanol stress, and a difference is observed between the profiles of Rnhsp70-1 and Rnhsp70-2 transcripts during heat stress.

Boštjan Černila, Bronislava Črešnar, and Katja Breskvar "Molecular characterization of genes encoding cytosolic Hsp70s in the zygomycete fungus Rhizopus nigricans," Cell Stress & Chaperones 8(4), 317-328, (1 October 2003).<0317:MCOGEC>2.0.CO;2
Received: 19 February 2003; Accepted: 1 June 2003; Published: 1 October 2003

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