Boliviana floridensis n. sp. (Apicomplexa: Eugregarinida: Gregarinidae) is described on the basis of morphology and life history in its natural host, the grasshopper Romalea microptera (Orthoptera: Romalidae) from south Florida, U.S.A. Trophozoites are slender with a globular epimerite. Protomerites are very broadly ovoid in early trophozoites, becoming deltoid in later stages. Deutomerites are deeply obovoid in immature trophozoites, becoming narrowly obdeltoid in mature trophozoites. Association is precocious, biassociative, and caudofrontal, with the satellite protomerite engulfing the posterior end of the primite deutomerite to form an interlock. Gametocysts are spherical with a conspicuous hyaline coat that dehisces through a single, short, and wide sporoduct. Oocysts are elliptoid. In the laboratory at ca. 28°C, the life cycle is completed in 18–20 d.