A checklist, based on bibliographical records and original data from an extensive survey, is presented for the metazoan parasites of Galaxias maculatus (Jenyns, 1842) in Argentinean Patagonia. The checklist includes 33 metazoan parasite entries: 5 myxozoans, 12 digeneans, 2 monogeneans, 3 cestodes, 2 acanthocephalans, 7 nematodes, 1 copepod, and 1 bivalve. Gyrodactylus sp., Pomphorhynchus patagonicus Ortubay, Úbeda, Semenas and Kennedy, 1991, and Hysterothylacium patagonense Moravec, Urawa and Coria, 1997 are reported for the first time from G. maculatus in South America. Although this checklist enlarges the knowledge of G. maculatus parasites, a large portion of Patagonia remains unexplored, and many of its lakes and rivers have never been surveyed for parasites, especially in the southern part of the region.