The invasive nematode Anguillicoloides crassus parasitizes the swim bladder of eels, including that of the American eel, Anguilla rostrata. Despite its potential devastating effects on eel populations, however, its intermediate host(s) is/are unknown in North America. The goal of this study was to determine whether An. crassus could infect copepods collected in the wild from the Goose Creek Reservoir in South Carolina, U.S.A., where An. crassus is known to occur. Cyclopoid copepods, identified morphologically as belonging to the Acanthocyclops robustus–americanus–vernalis species complex, were successfully experimentally infected by second-stage larvae (L2s) of An. crassus from eels captured in South Carolina, U.S.A. One infected copepod was identified as Ac. americanus via sequencing of a 251-base pair (bp) portion of mitochondrial cytochrome oxidase I DNA. For each infection experiment, 30 to 60 copepods were exposed for 24 hr to L2s at either 21°C or 26°C and maintained at their respective exposure temperatures to monitor larval development until larvae molted to the L3 stage. More than 85% of the exposed copepods showed L2s in their intestine or hemocoel at 24 hr postexposure. Larvae molted into the L3 stage in 7 d at 26°C and 12 d at 21°C. Based on these results, species within the Acanthocyclops vernalis–robustus–americanus complex are implied to be natural intermediate hosts of An. crassus in North America.
You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither BioOne nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the BioOne website.
Vol. 83 • No. 2