Forty-five specimens of Inanga, Galaxias maculatus, were collected during December 2013 and January 2014 from the Lingue River in southern Chile and examined for eukaryotic parasites. Thirty-seven (82.2%) of the fish were infected with 1 to 4 taxa with a mean of 1.7 taxa per host. All parasites detected in G. maculatus were recorded for the first time in the Lingue River, and cysts of Gordiids were determined for the first time in this host species. Also recorded for the first time in Chile were Stephanoprora uruguayense metacercariae and Diplodon chilensis glochidia on host gills, as well as plasmodia of Myxobolus sp. in the intestinal wall, stomach, and liver of G. maculatus. Other parasites recorded were Trichodina sp., Myxobolus magellanicus, Contracaecum sp., and Acanthocephalus tumescens. The prevalence (53.3%) and mean intensity (31.1 ± SD 95.9 cyst/host) of infection by cysts of Gordiids were the highest among the identified parasites.