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1 January 2000 Use and Advantages of Ethanol Solution of Alizarin Red S Dye for Staining Bone in Fishes
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Abstract

A solution of 75% ethyl alcohol (ETOH) and alizarin red S can be substituted for KOH-alizarin red S to stain bone. ETOH-alizarin, unlike KOH-alizarin, does not damage muscle or cartilage. ETOH-alizarin and acidified alcian blue (a cartilage stain) can be used in any order and as often as required to stain bone and cartilage in a single preparation that has not been exposed to KOH-alizarin or trypsin, remembering that the acidic effects of the alcian blue solution should be neutralized to prevent decalcification of weakly ossified elements.

The American Society of Ichthyologists and Herpetologists
Victor G. Springer and G. David Johnson "Use and Advantages of Ethanol Solution of Alizarin Red S Dye for Staining Bone in Fishes," Copeia 2000(1), 300-301, (1 January 2000). https://doi.org/10.1643/0045-8511(2000)2000[0300:UAAOES]2.0.CO;2
Accepted: 28 May 1999; Published: 1 January 2000
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