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13 January 2014 Development of a TILLING resource in durum wheat for reverse- and forward-genetic analyses
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Abstract

A durum wheat TILLING (targeting induced local lesions in genomes) population of 2601 M3 families was developed from cv. Svevo using ethyl methanesulfonate as a chemical mutagen. The entire M3 population was field-grown for phenotypic evaluations. Despite the polyploid nature of the wheat genome, a preliminarily phenotypic screening showed a high frequency of morphological alterations (∼22%); specific phenotyping for seed morphology was undertaken. Furthermore, a reverse-genetics experiment was performed on DNA collected from M2 leaves for the homoeologous genes SBEIIa-A and SBEIIa-B involved in starch metabolism. One non-sense mutation for both genes was identified; specific crosses are planned in order to pyramid the two mutations.

© CSIRO 2014
R. Bovina, A. Brunazzi, G. Gasparini, F. Sestili, S. Palombieri, E. Botticella, D. Lafiandra, P. Mantovani, and A. Massi "Development of a TILLING resource in durum wheat for reverse- and forward-genetic analyses," Crop and Pasture Science 65(1), (13 January 2014). https://doi.org/10.1071/CP13226
Received: 28 June 2013; Accepted: 1 October 2013; Published: 13 January 2014
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