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11 August 2017 Development of an ultrasound-assisted extraction method for the rapid quantification of seed carotenoid content in oilseed rape
Lei Xue, Fang Wei, Guizhen Gao, Guixin Yan, Weilin Song, Biyun Chen, Kun Xu, Hong Chen, Xiaoming Wu
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Abstract

The selection of cultivars with high carotenoid content represents a novel goal in the genetic improvement of oilseed rape. The successful development of an efficient method for the quantification of carotenoids in oilseed rape is a prerequisite for this breeding initiative. In the present study, ultrasound-assisted extraction was combined with a spectrophotometric method to quantify seed carotenoid levels in oilseed rape. The key variables were optimised as follows: the solvent used was a 1 : 1 (v : v) mixture of petroleum ether and acetone, the solid to solvent concentration was 0.08 g/mL, the ultrasound frequency and power were set at 45/100 (kHZ/w), and three extractions of 15 min each were performed. Compared with the conventional solvent extraction method, the time required for extraction using the ultrasound-assisted extraction method was significantly shortened (by 24 times), and the reproducibility was also remarkably improved. These results indicate that ultrasound-assisted spectrophotometric quantification is a rapid, accurate, simple and low cost tool for the screening and selection of rapeseed cultivars with high carotenoid content.

© CSIRO 2018
Lei Xue, Fang Wei, Guizhen Gao, Guixin Yan, Weilin Song, Biyun Chen, Kun Xu, Hong Chen, and Xiaoming Wu "Development of an ultrasound-assisted extraction method for the rapid quantification of seed carotenoid content in oilseed rape," Crop and Pasture Science 69(1), 48-54, (11 August 2017). https://doi.org/10.1071/CP16351
Received: 22 September 2016; Accepted: 1 July 2017; Published: 11 August 2017
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KEYWORDS
quality breeding
spectrophotometric quantification
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