The spore output of 26 selected species of 11 genera of Lejeuneaceae was investigated by the direct artificial counting method. The mean spore output per capsule ranges from 257 in Cololejeunea magnilobula to 5038 in Ptychanthus striatus. Compared with other leafy liverwort families, Lejeuneaceae have a much lower and more stable spore output. Out of eight species of Ptychanthoideae, Acrolejeunea pusilla is the only species with a mean spore output of less than 1000 spores per capsule. Acrolejeunea securifolia, Cheilolejeunea larsenii and Cololejeunea furcilobulata are reported for China for the first time.