Two new species of the salamandrid genus Pachytriton are described from Anlui and Guangxi, China, based on molecular and morphological analyses. One of them from Huangshan, Anhui, has smaller body, longer limbs, and larger eye than the remaining congeners. The other from northeastern Guangxi possesses a larger body and head, and longer snout and tooth series than the remaining congeners. Also, we have found that two individuals of Pachytriton obtained from pet shops in Japan can represent an unnamed species, but do not describe them as new because we have no exact information on their original localities. The distribution patterns and ecological relationships among species of Pachytriton are discussed.