Three species/subspecies of toads (Bufo japonicus japonicus, B. j. formosus, and B. torrenticola) show parapatric/sympatric patterns of distribution in Japan, and usually they are morphologically indistinguishable at early developmental stages. For quick and easy genetic identification of these three toads, we validated LAMP assay. We designed taxon-specific primers for LAMP assay on mitochondrial cytochrome b region, and these primer sets accurately assigned DNA samples derived from three toads to each taxon. Due to its instancy and simplicity, LAMP method could be a useful diagnostic tool to distinguish taxa which resemble each other.
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1 February 2016
Development and Evaluation of Loop-Mediated Isothermal Amplification (LAMP) Assay for Quick Identification of Three Japanese Toads
Koshiro Eto,
Masafumi Matsui,
Kanto Nishikawa,
Takashi Haramura
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Current Herpetology
Vol. 35 • No. 1
February 2016
Vol. 35 • No. 1
February 2016
Bufo
cytochrome b
LAMP
species identification