This paper reports on the knowledge and useful applications of various fungi by the Hotï, a recently contacted Native American society inhabiting the Sierra Maigualida region of the Venezuelan Amazon. At least 31 folk taxa of fungi are esteemed as sources of nutrition, powerful media for hunting magic, protective charms against black magic, human medicinal agents, or body adornments. Even though the results presented here probably do not exhaust the total number of species known and used by them, they nevertheless suggest that the Hotï constitute one of the rare mycophilic societies in the Venezuelan Amazon. Further research is needed in order to obtain a more complete picture of the complexity of human-fungi relationships in this society.