The wild bee fauna of six natural protected areas (NPAs) in Yucatan State, Mexico, are described and analyzed. A total of 130 bee species was recorded belonging to 50 genera and five families, of which 32 are new records for Yucatan State. The genera with the highest species richness were Megachile (20 species), Lasioglossum (12 species), Coelioxys (11 species, most of these are cleptoparasites of Megachile), Ceratina (9 species) and Augochlora (8 species). Species richness by NPA was 30 species in El Palmar; 55 in Dzibilchaltún; 58 in Kabah; 60 in Dzilam; 69 in Tabi; and 79 in Yalahau. Bee faunal composition in the NPAs, biogeographic affinities and the importance of wild bee conservation in the region are discussed.