Taxonomic research on animals is an important topic. In particular, closely related species are difficult to differientiate by morphological descriptions. The Lycosidae, commonly called wolf spiders, is one of the largest spider families, and species separation needs to be supported by molecular studies.
In morphological studies of spider specimens of the genus Alopecosa only certain mature specimens could be diagnosed to species. In molecular studies, both mature and juvenile specimens were investigated and mitochondrial COI gene sequences were analyzed using MEGA6 software. In total, high quality sequences were obtained for 76 specimens and a phylogenetic tree was contructed and evaluated, with a 1000 bootstrap repetetition. As a result four Alopecosa species, Alopecosa aculeata (Clerck, 1757), A. kuntzi Denis, 1953, A. albofasciata (Brullé, 1832) and A. accentuata (Latreille, 1817), were identified and their DNA sequences recorded in Gen bank. Of these species Alopecosa kuntzi was newly added to the list of known Turkish spider species.