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1 August 2010 Molecular Phylogeny of the Genus Dactylopius (Hemiptera: Dactylopiidae) and Identification of the Symbiotic Bacteria
S. T. Ramírez-Puebla, M. Rosenblueth, C. K. Chávez-Moreno, M. C. Catanho Pereira De Lyra, A. Tecante, E. Martínez-Romero
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Abstract

Phylogenetic analyses, from polymerase chain reaction (PCR)-amplified 12S rRNA and 18S rRNAgene sequences from cochineal insects of the genus Dactylopius present in Mexico, showed that D. ceylonicus, D. confusus, and D. opuntiae are closely related. D. coccus constitutes a separate clade, and D. tomentosus is the most distantly related. Bacterial 16S rRNA sequences from all the Dactylopius species sampled showed a common β-proteobacteria, related to Azoarcus, also found in eggs and in bacteriocytes in D. coccus. We propose the name “Candidatus Dactylopiibacterium carminicum” for this endosymbiont. Other bacterial sequences recovered from the samples were close to those from soil or plant associated bacteria, like Massilia, Herbaspirillum, Acinetobacter, Mesorhizobium, and Sphingomonas, suggesting a possible horizontal transmission from Cactaceae plant sap to Dactylopius spp. during feeding. This is the first molecular analysis of Dactylopius species and of their associated bacteria.

© 2010 Entomological Society of America
S. T. Ramírez-Puebla, M. Rosenblueth, C. K. Chávez-Moreno, M. C. Catanho Pereira De Lyra, A. Tecante, and E. Martínez-Romero "Molecular Phylogeny of the Genus Dactylopius (Hemiptera: Dactylopiidae) and Identification of the Symbiotic Bacteria," Environmental Entomology 39(4), 1178-1183, (1 August 2010). https://doi.org/10.1603/EN10037
Received: 8 February 2010; Accepted: 13 May 2010; Published: 1 August 2010
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