Translator Disclaimer
1 December 2012 Development of an Early-Tier Laboratory Bioassay for Assessing the Impact of Orally-Active Insecticidal Compounds on Larvae of Coccinella septempunctata (Coleoptera: Coccinellidae)
Fernando Álvarez-Alfageme, Zoltan Pálinkás, Franz Bigler, Jörg Romeis
Author Affiliations +
Abstract

Early-tier studies are the initial step in the environmental risk assessment of genetically engineered plants on nontarget arthropods. They are conducted in the laboratory where surrogate species are exposed to higher concentrations of the arthropod-active compound than those expected to occur in the field. Thus, early-tier tests provide robust data and allow to make general conclusions about the susceptibility of the surrogate to the test substance. We have developed an early-tier test for assessing the toxicity of orally-active insecticidal compounds to larvae of the ladybird beetle Coccinella septempunctata L. (Coleoptera: Coccinellidae). Using potassium arsenate and the protease inhibitor E-64 as model compounds, we validated the bioassay set-up for C. septempunctata. Sucrose solution containing the test compound was offered to larvae for 24 h on the first day of each of its four larval instars. Subsequently, larvae were fed ad libitum with Ephestia kuehniella (Zeller) (Lepidoptera: Pyralidae) eggs. Both compounds negatively affected C. septempunctata larval survival and development, and adult weight, indicating that the bioassay setup was able to detect dietary effects of insecticidal substances on the ladybird. Power analyses revealed that sample sizes of 20 or 45 per treatment are sufficient to detect 50 or 20% differences between the control and treatment groups, respectively, for the various measurement endpoints.

© 2012 Entomological Society of America
Fernando Álvarez-Alfageme, Zoltan Pálinkás, Franz Bigler, and Jörg Romeis "Development of an Early-Tier Laboratory Bioassay for Assessing the Impact of Orally-Active Insecticidal Compounds on Larvae of Coccinella septempunctata (Coleoptera: Coccinellidae)," Environmental Entomology 41(6), 1687-1693, (1 December 2012). https://doi.org/10.1603/EN12032
Received: 31 January 2012; Accepted: 1 August 2012; Published: 1 December 2012
JOURNAL ARTICLE
7 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top