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1 April 2015 Evaluation of Reference Genes for RT-qPCR in Tribolium castaneum (Coleoptera: Tenebrionidae) Under UVB Stress
Wen Sang, Li He, Xiao-Ping Wang, Keyan Zhu-Salzman, Chao-Liang Lei
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Abstract

Reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) has become a widely used technique to quantify gene expression. It is necessary to select appropriate reference genes for normalization. In the present study, we assessed the expression stability of seven candidate genes in Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) irradiated by ultraviolet B (UVB) at different developmental stages for various irradiation time periods. The algorithms of geNorm, NormFinder, and BestKeeper were applied to determine the stability of these candidate genes. Ribosomal protein genes RpS3, RpL13A, and β-actin gene (ActB) showed the highest stability across all UVB irradiation time points, whereas expression of other normally used reference genes, such as those encoding the β-tubulin gene TUBB and the E-cadherin gene CAD, varied at different developmental stages. This study will potentially provide more suitable reference gene candidates for RT-qPCR analysis in T. castaneum subjected to environmental stresses, particularly UV irradiation.

© The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com
Wen Sang, Li He, Xiao-Ping Wang, Keyan Zhu-Salzman, and Chao-Liang Lei "Evaluation of Reference Genes for RT-qPCR in Tribolium castaneum (Coleoptera: Tenebrionidae) Under UVB Stress," Environmental Entomology 44(2), 418-425, (1 April 2015). https://doi.org/10.1093/ee/nvv010
Received: 6 June 2014; Accepted: 22 January 2015; Published: 1 April 2015
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KEYWORDS
Reference gene
RT-qPCR
Tribolium castaneum
UVB
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